Analysis of thiosemi carbazon complexes effects on UCA1 lncRNA and AKT target, gene expression alternations, regulating PI3K/ AKT signaling pathway in Jurkat E6.1 Acute Lympholastic Leukemia cell line

Background and Aim:Leukemia usually begins in the bone marrow and leads to the production of a large number of abnormal white blood cells.The goal of this study was to investigate changes on UCA1 lncRNA and AKT target, gene expression alternations, regulating PI3K/ AKT signaling pathway in Jurkat E6.1 Acute Lympholastic Leukemia cell line under treatment with thiosemicarbazone complexes(nickel). Materials and Methods: First, thiosemicarbazones complex Ni and 6MP was provided in different concentrations(0.5,1,2,5macromolecular)and(1,5,10,25macromolecular) and the jurkat E.6.1 Cancer cells were treated with mentioned doses at (24-48-72hours)after cell passage. Next RNA extraction and cDNA synthesis were performed and the expression of UCA1 and AKT gene were appraised by Real Time PCR. Finally, the results were analyzed by Rest Software. Results:UCA1 showed a significant decrease during 24hours of treatment with 6mp at concentrations(1,5,10and25macromolecular)(P<0.001).In nickel,a significant decrease at 72hours was observed at concentrations(2and 5macromolecular).in the AKT in treatment with 6mp at 24hours At concentrations(5,10and 25macromolecular)And all concentrations(1,5,10and25macromolecular)at 72hours showed a significant decrease(P<0.001).In nickel at concentrations(0.5,1,2and5macromolecular)at 24hours Decreased expression was observed,This decrease is not statistically significant at a concentration of 0.5μM.at concentrations(2and 5macromolecular)Significant reductions at 48and72hours were observed(P<0.001). Conclusion:UCA1 and AKT expression changes after treatment with 6mp and nickel depend on drug timing and concentration.UCA1 in 6MP treatment at 25μM and 24hours,in treatment with nickel at 5μM and 72hours , AKT in 6mp treatment at 25μM and 72hours , In treatment with nickel at 5μM and 24hours,It had the highest effect on the cell due to gene expression.