肠道菌群失调的幼儿为肠聚集性大肠杆菌携带者

M. Makarova, L. Suzhaeva, L. Kaftyreva
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引用次数: 1

摘要

的目标。研究肠道生态失调患儿肠聚集组致泻性大肠杆菌的流行情况。材料和方法。采用PCR方法对393例2岁以下儿童粪便中分离的511株大肠杆菌的毒力因子进行了研究。采用圆盘扩散法测定抗菌药物敏感性,结果解释-按临床推荐进行。微生物对抗菌制剂敏感性测定,2015。结果:共检出23株肠聚集性大肠杆菌(EAggEC)。所有菌株均有aaf基因编码聚集粘附菌毛,另外4个基因(aggR、ast、aap、aatA)以不同组合编码毒力因子EAggEC。19株(87%)对抗菌药物不敏感。通过检测CTX-M遗传家族的扩展谱β -内酰胺酶(ESBL)和AmpC类头孢菌素酶的产生来确定对扩展谱头孢菌素的耐药性。结论。该研究结果表明,6%的肠道生态失调儿童是EAggEC携带者,这证明了不仅在腹泻综合征患者中检测EAggEC菌株,而且在肠道生态失调患者中检测EAggEC菌株的必要性。
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YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI
Aim. Study the prevalence of diarrhea-genic E. coli of the enteroaggregative group in children with intestine dysbiosis. Materials and methods. PCR method was used to study virulence factors in 511 strains of E. coli isolated during bacteriologic study of feces samples from 393 children aged less than 2 years. Sensitivity to antibiotics was determined by disc-diffusion method, results interpretation - according to clinical recommendations Determination of sensitivity of microorganisms to antimicrobial preparations, 2015. Results. 23 enteroaggregative E. coli strains were identified (EAggEC). All the strains had aaf gene coding aggregative-adhesion fimbriae and 4 other genes (aggR, ast, aap, aatA) in various combinations coding virulence factors EAggEC. 19 strains (87%) were not sensitive to antimicrobial preparations. Resistance to extended spectrum cephalosporins was determined by the production of extended spectrum beta-lactamase (ESBL) of CTX-M genetic family and AmpC cephalosporinase. Conclusion. Results of the study have shown that 6% of children with intestine dysbiosis are EAggEC carriers, that gives evidence on the necessity of detection of EAggEC strains - a novel group of diarrhea-genic E. coli not only in patients with diarrhea syndrome, but also using intestine dysbiosis.
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来源期刊
Zhurnal mikrobiologii, epidemiologii, i immunobiologii
Zhurnal mikrobiologii, epidemiologii, i immunobiologii Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
1.40
自引率
0.00%
发文量
51
审稿时长
8 weeks
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