促性腺激素释放激素类似物(GnRHa)免疫对罗非鱼性腺转录组和蛋白质组的影响。

Rui Wang, Luting Wen, Huawei Ma, Min Lv, Zhong Chen, Xuesong Du, Yong Lin, Huizan Yang
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引用次数: 2

摘要

促性腺激素释放激素(Gonadotropin releasing hormone, GnRH)在脊椎动物生殖过程中起着重要的调节作用。研究表明,免疫GnRHa可诱导罗非鱼性不育。为了探究其背后的机制,本研究采用RNA-seq和数据独立获取(DIA)技术对GnRHa免疫罗非鱼性腺的转录组和蛋白质组进行了研究。共鉴定出644个差异表达基因(80个上调,564个下调)和1150个差异表达蛋白(351个上调,799个下调)。在转录组学和蛋白质组学分析中,有209个基因具有一致的差异表达模式,其中9个基因表达上调,200个基因表达下调,表明GnRHa免疫抑制了性腺基因表达。下调的基因主要参与单生物过程、结合过程、细胞过程、代谢过程和催化活性的功能,并与ecm受体相互作用、局灶黏附、心肌收缩和氧化磷酸化等途径相关。参与GnRH信号通路的6个差异表达基因的表达均下调。此外,筛选了GnRHa免疫后与性腺发育相关的几个重要功能基因。本研究在分子水平上证实了GnRHa对罗非鱼性腺发育影响相应基因的表达,为阐明GnRHa免疫的机制奠定了基础。
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Effects of gonadotropin-releasing hormone analog (GnRHa) immunization on the gonadal transcriptome and proteome of tilapia (Oreochromis niloticus).
Gonadotropin releasing hormone (GnRH) plays an important role in the regulation of vertebrate reproduction. Studies have shown that immunization against GnRHa can induce sexually sterile tilapia. To explore the mechanism behind this, in this study, RNA-seq and data-independent acquisition (DIA) techniques were used to study the transcriptome and proteome of the gonad of tilapia immunized with GnRHa. 644 differentially expressed genes (80 upregulated and 564 downregulated) and 1150 differentially expressed proteins (351 upregulated and 799 downregulated) were identified. There were 209 genes with consistent differential expression patterns in the transcriptomic and proteomic analyses, of which 9 were upregulated and 200 downregulated, indicating that the gonad gene expression was inhibited by GnRHa immunization. The downregulated genes were particularly involved in the functions of single-organism process, binding, cellular process, metabolic process and catalytic activity, and associated with the pathways including ECM-receptor interaction, focal adhesion, cardiac muscle contraction and oxidative phosphorylation. The expression of six differentially expressed genes involved in the GnRH signaling pathway was all downregulated. In addition, several important functional genes related to gonadal development after GnRHa immunization were screened. This study confirmed the expression of corresponding genes was affected by GnRHa on the gonad development in tilapia at the molecular level, and laid a foundation for elucidating the mechanism of GnRHa immunization.
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