微卫星标记在多倍体鉴定中的应用——以六倍体竹子为例

IF 1.1 4区 农林科学 Q3 FORESTRY Silvae Genetica Pub Date : 2020-01-01 DOI:10.2478/sg-2020-0013
R. Meena, M. Bhandari, H. Ginwal
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引用次数: 3

摘要

微卫星标记是植物遗传资源鉴定或群体遗传分析最有价值的工具。由于它们是共显性和等位基因标记,因此在多倍体物种中利用它们尚存疑问。在这种情况下,通常将微卫星标记作为显性标记进行分析。目前的研究表明,微卫星标记虽然失去了共显性的优势,但由于每个位点有大量可重复的等位基因,因此仍然是多倍体物种基因分型的有力工具。利用17对多态性SSR引物对六倍体竹(Dendrocalamus hamiltonii) 19个亚群体进行了基因分型研究。其中10条引物在二倍体物种中呈现典型的微卫星标记带型,其余7条引物呈现异常带型,即每个基因型每个位点都有2条以上带。在这种情况下,基因分型数据通常通过考虑显性标记来分析。鉴于这些事实,数据以两种方式分析为主导和共主导。首先将17个引物标记为非等位基因数据进行分析;将10对标准带型引物作为等位基因数据进行分析,并对结果进行比较。UPGMA聚类分析和遗传结构分析表明,两组数据得到的结果非常相似,因此SSR标记可以作为优势标记用于多倍体物种的鉴定。该研究对扩大SSR标记的应用范围和多倍体物种的研究具有重要意义。
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Usage of microsatellite markers for characterization of polyploids: a case study in reference to hexaploid bamboo species
Abstract Microsatellite markers are most valuable tools for characterization of plant genetic resources or population genetic analysis. Since they are codominant and allelic markers, utilizing them in polyploid species remained doubtful. In such cases, microsatellite markers are usually analyzed by treating them as dominant marker. In the current study, it has been showed that despite of losing the advantage of co-dominance, microsatellite markers are still powerful tool for genotyping of polyploid species because of availability of large number of reproducible alleles per locus. It has been studied by genotyping of nineteen sub populations of Dendrocalamus hamiltonii (hexaploid bamboo species) with seventeen polymorphic SSR primer pairs. Among these, ten primers gave typical banding pattern of microsatellite marker as expected in diploid species but rest seven gave unusual pattern i.e. more than two bands per locus per genotype. In such case genotyping data are generally analyzed by considering as dominant markers. Given these facts, data were analyzed in both ways as dominant and codominant. All the seventeen primer were first scored as non-allelic data and analyzed; later ten primer pairs giving standard banding pattern were analyzed as allelic data and the results were compared. The UPGMA clustering and genetic structure showed that results obtained with both the data sets were very similar, and therefore the SSR marker could be utilized to characterize polyploid species by considering them as dominant marker. The study is highly useful to widen the scope of SSR markers applications and beneficial to the researchers dealing with polyploid species.
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来源期刊
Silvae Genetica
Silvae Genetica 农林科学-林学
CiteScore
2.20
自引率
10.00%
发文量
10
审稿时长
3 months
期刊介绍: Silvae Genetica is an international peer reviewed journal with more than 65 year tradition and experience in all fields of theoretical and applied Forest Genetics and Tree breeding. It continues "Zeitschrift für Forstgenetik und Forstpflanzenzüchtung" (Journal of Forest Genetics and Forest Tree Breeding) founded by W. LANGNER in 1951.
期刊最新文献
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