{"title":"氨氧化细菌富集及微生物多样性高通量测序分析","authors":"Renda Yao, Mengyu Yu Yi Liu Hong Yang, H. Shi","doi":"10.12783/ISSN.1544-8053/14/1/10","DOIUrl":null,"url":null,"abstract":"A fully automatic miniature fermenter was operated to enrich ammoniaoxidizing bacteria (AOB) using the activated sludge obtained from the A2/O process in a wastewater treatment plant (WWTP) as the seed sludge. The results revealed that the AOB community can be enriched by a continuous ammonia feeding mode for intermittent operation, resulting in the appropriate regulation of free ammonia (FA) and free nitrous acid (FNA) levels. The maximum ammonia oxidation rate was over 170 mg/ (L·h) and nitrite accumulation ratio over 80%. High-throughput sequencing showed that Nitrosomonas belonging to AOB, were highly enriched after 27 days, having increased from 0.24% to 53.03%.","PeriodicalId":17101,"journal":{"name":"Journal of Residuals Science & Technology","volume":"7 1","pages":"77-84"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Enrichment of Ammonia-Oxidizing Bacteria and Microbial Diversity Analysis by High-Throughput Sequencing\",\"authors\":\"Renda Yao, Mengyu Yu Yi Liu Hong Yang, H. Shi\",\"doi\":\"10.12783/ISSN.1544-8053/14/1/10\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A fully automatic miniature fermenter was operated to enrich ammoniaoxidizing bacteria (AOB) using the activated sludge obtained from the A2/O process in a wastewater treatment plant (WWTP) as the seed sludge. The results revealed that the AOB community can be enriched by a continuous ammonia feeding mode for intermittent operation, resulting in the appropriate regulation of free ammonia (FA) and free nitrous acid (FNA) levels. The maximum ammonia oxidation rate was over 170 mg/ (L·h) and nitrite accumulation ratio over 80%. High-throughput sequencing showed that Nitrosomonas belonging to AOB, were highly enriched after 27 days, having increased from 0.24% to 53.03%.\",\"PeriodicalId\":17101,\"journal\":{\"name\":\"Journal of Residuals Science & Technology\",\"volume\":\"7 1\",\"pages\":\"77-84\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Residuals Science & Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12783/ISSN.1544-8053/14/1/10\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Residuals Science & Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12783/ISSN.1544-8053/14/1/10","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Enrichment of Ammonia-Oxidizing Bacteria and Microbial Diversity Analysis by High-Throughput Sequencing
A fully automatic miniature fermenter was operated to enrich ammoniaoxidizing bacteria (AOB) using the activated sludge obtained from the A2/O process in a wastewater treatment plant (WWTP) as the seed sludge. The results revealed that the AOB community can be enriched by a continuous ammonia feeding mode for intermittent operation, resulting in the appropriate regulation of free ammonia (FA) and free nitrous acid (FNA) levels. The maximum ammonia oxidation rate was over 170 mg/ (L·h) and nitrite accumulation ratio over 80%. High-throughput sequencing showed that Nitrosomonas belonging to AOB, were highly enriched after 27 days, having increased from 0.24% to 53.03%.
期刊介绍：
The international Journal of Residuals Science & Technology (JRST) is a blind-refereed quarterly devoted to conscientious analysis and commentary regarding significant environmental sciences-oriented research and technical management of residuals in the environment. The journal provides a forum for scientific investigations addressing contamination within environmental media of air, water, soil, and biota and also offers studies exploring source, fate, transport, and ecological effects of environmental contamination.
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