人胎盘碱性磷酸酶在交叉流微滤聚偏二氟乙烯膜上的共价固定化。活性与[底物]动力学行为

Q4 Chemical Engineering 分子催化 Pub Date : 1994-09-27 DOI:10.1016/0304-5102(94)00079-4

M.G. Roig , J.F. Bello , S. Rodriguez , J.M. Cachaza , J.F. Kennedy

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引用次数: 1

摘要

将人胎盘碱性磷酸酶化学固定在以1-羰基咪唑基团为化学标记的聚偏二氟乙烯(PVDF)制成的亲水性交叉流微滤膜上，并对其进行了物理化学表征。(第93卷，85-104卷))在没有扩散约束的情况下，通过非线性回归和使用模型判别的F统计检验，以图形和数值方式分析了内在动力学行为(速率与底物浓度)，假设底物浓度的最小合理速率方程为2:2度。根据所发现的结果，推测了催化动力学作用的机理。

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Human placental alkaline phosphatase covalently immobilised on a cross-flow microfiltration polyvinylidene difluoride membrane Part II. Activity vs. [substrate] kinetic behaviour

Human placental alkaline phosphatase was chemically immobilized on a hydrophilic cross-flow microfiltration membrane made of polyvinylidene difluoride (PVDF) chemically labelled with 1-carbonylimidazole groups and characterized physicochemically (in Part I, J. Mol. Catal. (Vol. 93, 85–104)) The intrinsic kinetic behaviour (rate vs. substrate concentration) in the absence of diffusional constraints was analysed graphically and numerically, by non-linear regression and use of the F statistical test for model discrimination, postulating a minimum rational rate equation of 2:2 degree in substrate concentration. According to the results found, a mechanism for the catalytic kinetic action has been postulated.

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