紫外- c和有机酸处理对高碳水化合物糖果中真菌的影响

Ji-Eun Lee, Xiaotong Xu, S. Jeong, W. Kang, S. Ryu, Yehum Kim, D. Ahn
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摘要

本研究的目的是鉴定在糖果产品的储存和分销过程中降解产品价值的真菌,并研究有机酸和UV-C处理对高碳水化合物产品的抗真菌作用。根据ITS1和ITS4序列的同源性,从变质的高碳水化合物糖果中分离到的真菌分别为Wallemia sp.、Aspergillus sp. 1和Aspergillus sp. 2。分别用乙酸、柠檬酸、乳酸和马来酸对分离得到的真菌进行抑菌活性测定。结果证实,0.2 M和0.35 M乙酸分别抑制了wallleia sp.和Aspergillus sp. 2的生长。另外,根据UV-C照射时间对抗真菌效果进行了确认,在照射30 min时显示了wallleia sp.和Aspergillus sp. 2的生长抑制效果,在照射40 min时显示了Aspergillus sp. 1的生长抑制效果。对未经处理和0.35 M醋酸处理的高碳水化合物糖果进行感官评价的结果显示,其口感、颜色、异常口感、硬度和质地,但酸味和气味有显著差异。通过以上研究,证实了有机酸和UV-C辐照处理对产品真菌生长的抑制作用,有望应用于销售过程中关注真菌发生的糖果。
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Effect of Ultraviolet-C and Organic Acid Treatment on Fungi Isolated from High Carbohydrate Confectionery
The purpose of this study was to identify fungi that degrade product value during the storage and distribution of confectionery products, and to investigate the antifungal effect of organic acid and UV-C treatments on high carbohydrate products. Fungi isolated from spoiled high carbohydrate confectionery were identified as Wallemia sp., Aspergillus sp-1 and Aspergillus sp-2 depending on homologies with ITS1 and ITS4 sequences. The isolated fungi were assayed for antifungal activity by treatment with acetic acid, citric acid, lactic acid or maleic acid. As a result, it was confirmed that the growth of Wallemia sp. and Aspergillus sp-2 was suppressed by treatment with 0.2 M and 0.35 M acetic acid, respectively. In addition, as a result of confirming the antifungal effect according to the UV-C irradiation time, the growth inhibitory effects of Wallemia sp. and Aspergillus sp-2 were shown in irradiation for 30 min and the growth inhibitory effect of Aspergillus sp-1 was shown in irradiation for 40 min. The result of the sensory evaluation of the untreated and 0.35 M acetic acid-treated high carbohydrate confectionery, there were not significant changes in taste, color, abnormal taste, hardness and texture, but there were significant differences in sour taste and smell. As a result of the above study, the effect of inhibiting fungi growth on the product by treatment with organic acid and UV-C irradiation was confirmed, and it is expected to be used in confectionery that were concerned about the occurrence of fungi in the distribution process.
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