沙鼠抗氧化活性研究。萃取物及含硫化合物

B. Bayrak, R. Yanardağ
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引用次数: 0

摘要

目的:研究沙蚕。生长在土耳其的地中海东部和东南部地区,在这些地区通常被用作食用植物。本研究的目的是测定不同种类的黄芪的抗氧化活性。提取物和一些含硫化合物。材料与方法:本研究考察了黄芪水提液、乙醇提液和乙酸乙酯提液的抗氧化活性。和硫化合物(α-硫辛酸、半胱氨酸、半胱氨酸、二烯丙基硫醚、谷胱甘肽、同型半胱氨酸、n -乙酰半胱氨酸、维生素U和1,4-二硫代赤四糖醇)在若干抗氧化试验中进行了评估。这些试验包括还原能力、铜离子还原抗氧化能力、2,2'-氮基-双(3-乙基苯并噻唑-6-磺酸)自由基清除能力、2,2-二苯基-1-苦基肼基自由基清除能力、N,N-二甲基-4-苯二胺二盐酸自由基清除能力、超氧阴离子自由基清除能力、羟基自由基清除能力、金属螯合活性和铁离子还原抗氧化能力。研究了各提取物的植物化学特征及总酚和总黄酮含量。结果:各提取物及含硫化合物的抗氧化活性均随浓度的增加而增加,其中乙酸乙酯提取物的抗氧化活性最高,而水提物的抗氧化活性最低。二烯丙基硫化物具有最高的抗氧化活性,而维生素U具有最低的抗氧化活性。结果表明,黄芪提取物均具有明显的抗氧化活性。含硫化合物具有抗氧化活性。结论:该菌是一种特殊大肠杆菌。本研究中所研究的含硫化合物可以被认为是抗氧化剂的来源。
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Antioxidant Activities of Eremurus spectabilis M. Bieb. Extracts and Sulfur Compounds
Objective: Eremurus spectabilis M. Bieb. is grown in East and Southeast Mediterranean regions of Turkey, and is commonly used as an edible plant in these regions. The aim of the current study was to determine the antioxidant activities of different E. spectabilis M. Bieb. extracts and some sulfur compounds. Materials and Methods: In this study, the antioxidant activities of aqueous, ethanolic, and ethyl acetate extracts of E. spectabilis M. Bieb. and sulfur compounds (α-lipoic acid, cysteamine, cysteine, diallyl sulfide, glutathione, homocysteine, N-acetyl cysteine, vitamin U and 1,4-dithioerythritol) were assessed in several antioxidant tests. These tests included reducing power, cupric ion reducing antioxidant capacity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, 2,2-diphenyl-1-picrylhydrazyl radical scavenging, N,N-dimethyl-4-phenylenediamine dihydrochloride radical scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging, metal chelating activities and ferric ion reducing antioxidant power. The phytochemical profiles and total phenolic and flavonoid contents of all extracts were investigated. Results: It was found out that the antioxidant activities of all the extracts and sulfur compounds generally increased with concentration increases, the highest antioxidant activity was found in ethyl acetate extract, while the aqueous extract was found to have the lowest antioxidant activity. Diallyl sulfide was found to have the highest antioxidant activity, whereas vitamin U had the lowest antioxidant activity. It was observed that all extracts of E. spectabilis M. Bieb. and the sulfur compounds showed antioxidant activity. Conclusion: The E. spectabilis M. Bieb. and sulfur compounds investigated in this study could be considered as a source of antioxidants.
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