Salicola属的基因组测序初稿

L. Daoud, A. H. Brahim, Houda Hmani, Mouna Jlidi, M. Ali, S. Bejar, Naser Aliye Feto, Mamdouh Ben Ali
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引用次数: 0

摘要

Salicola sp.菌株SBJ9是一种新分离自突尼斯斯法克斯高盐湖的极端嗜盐细菌。它是一种需氧革兰氏阴性菌,呈杆菌状,可运动。菌株SBJ9在150 g/l (2.57 M) NaCl、35°C和pH 7条件下生长最佳,并能在高盐度条件下水解蛋白质和脂质。实际上,Salicola属只有两种(S. marasensis和S. salis)。本研究报道了Salicola属细菌的第一份基因组草图,以及获得和注释的不同序列和基因。Salicola sp.菌株SBJ9基因组序列为4.643.441 bp, G+C含量为60.52%。RAST服务器监测的注释显示65个RNA序列和3995个编码基因,分别包含255个和182个蛋白质代谢子系统和脂质子系统。SBJ9基因组序列的进一步分析将阐明Salicola菌株及其蛋白质对极高盐浓度的适应性,并为工业应用开发新的生物催化剂提供可能。
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First draft genome sequencing of the Salicola genus
Salicola sp. strain SBJ9 is an extremely halophilic bacterium newly isolated from a hypersaline lake in Sfax (Tunisia). It is an aerobic Gram negative bacterium, bacilli shaped and motile. The strain SBJ9 grows optimally at 150 g/l (2.57 M) NaCl, 35 °C and pH 7 and it is able to hydrolyze proteins and lipids at high salinities. Actually, Salicola genus contains only two species (S. marasensis and S. salis). This research reported the first draft genome of a bacterium belonging to Salicola and the different obtained and annotated sequences and genes. Salicola sp. strain SBJ9 genomic sequence contains 4.643.441 bp with a G+C content of 60.52%. The annotation monitored by the RAST server reveals 65 RNA sequences and 3995 coding genes including 255 and 182 sub-systems of protein metabolism and lipids, respectively. The further analysis of SBJ9 genomic sequence will clarify the ideas regarding the adaptation of the Salicola strains and their proteins to the extremely high salt concentrations and will permit the development of new biocatalyzers for industrial applications.
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