基于DNA的工业重要木材树种鉴定系统的开发方法

Tanzeem Fatima, A. Srivastava, V. S. Hanur, M. S. Rao
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摘要

商业非法贸易是对印度和工业化国家重要木材的最大威胁。因其木材和木制品而有价值的木材品种以无法分类学识别的掺假形式走私。发展DNA标记法对热带树种林木及其制品的原产地进行识别和控制,将有助于区分合法和非法采伐木材。因此,DNA条形码被认为是一种可靠的木材树种鉴定技术,可以确保采伐和交易的树木是同一树种/来源。DNA条形码的可用性增加了木材种类的数量,使得快速和准确的物种鉴定成为可能。这是第一次尝试将所有可用的木材条形码集合起来,作为印度木材品种的参考。本文描述了从植物材料采集、DNA提取、扩增、扩增区测序到条形码生成的整个DNA条形码过程。
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Approaches in development of DNA based identification system for industrially important timber species
Commercial illegal trade is the largest threat to important timbers in India and industrialized countries. Timber species valued for its wood and wood products are smuggled in the adulterated form that cannot be taxonomically identified. The development of DNA marker method to identify and control the origin of tree and tree products from tropical tree species would greatly contribute to distinguish legally from illegally harvested wood. Therefore, DNA barcoding has been anticipated as a reliable technique for wood species identification that can ensure that the tree harvested and traded are the same species/origin. The availability of DNA barcodes for increasing numbers of timber species allows rapid and accurate species identification. This is the first attempt to assemble all the timber barcodes which are available as a reference for the timber species of India. This paper describes whole DNA barcoding process from collection of plant material, to extract DNA and amplification as well as sequencing the amplified region to barcode generation.
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