黑兵蝇(Hermetia illucens)实验室分子鉴定及生活史研究

L. Ferdousi, N. Sultana, Maha A. Helal, N. Momtaz
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摘要

对孟加拉国生物地理区黑兵蝇(Hermetia illucens)进行了分子鉴定和生活史研究。测序结果和系统发育分析显示,BSF与GenBank中的H. illucens相似度为99-100%。雄性和雌性的平均生命周期分别为45.08±4.46d和46.15±4.12d。成年雌虫体长16.3±0.91mm,成年雄虫体长14.30±0.19 mm,体型小于雌虫。每窝蛋数为537.37±40.21枚,孵化周期为4.36±0.24 d。在29.40±1.77°C、相对湿度68.25±2.32%、光周期14:10 (L: d)条件下,幼虫、蛹、雄虫和雌虫的平均发育期分别为16.07±2.59、15.4±2.50、9.95±1.48和10.33±1.89 d。各发育阶段中,成熟幼虫体重最高(0.20±0.03 g)。
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Molecular identification and life cycle of Black Soldier fly (Hermetia illucens) in laboratory
Molecular identification and life cycle of the Black Soldier Fly (BSF), Hermetia illucens were carried out from the Bangladesh bio-geographical area. The sequencing result and phylogenetic analysis of BSF showed 99-100% similarity with H. illucens from GenBank. The average duration of life cycle of male and female were 45.08±4.46d and 46.15± 4.12d respectively. The adult female is 16.3±0.91mm long, whereas the adult male is 14.30±0.19 mm long and smaller than female. The number of eggs per clutch was 537.37±40.21 which hatched in 4.36±0.24 days. The mean duration of the developmental stages were 16.07±2.59, 15.4±2.50, 9.95±1.48 and 10.33±1.89 d for larva, pupa, male and female respectively, when cultured at 29.40±1.77° C, RH 68.25±2.32 %, 14:10 (L: D) photoperiod. The mature larval weight (0.20±0.03 g) was highest among other developmental stages.
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