忍冬素通过抑制EZH2/NF-κB信号通路减轻卵清蛋白诱导的小鼠哮喘。

IF 2.2 4区 农林科学 Q1 VETERINARY SCIENCES Experimental Animals Pub Date : 2024-05-03 Epub Date: 2023-11-10 DOI:10.1538/expanim.23-0068
Rui Dai, Yun Xiang, Rui Fang, Hai-Han Zheng, Qing-Song Zhao, Yan Wang
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引用次数: 0

摘要

哮喘是儿童呼吸系统最常见的慢性疾病,是由对常见抗原的异常免疫反应引起的。忍冬素通过靶向与哮喘相关的zeste同源物2增强子(EZH2)在其他炎症模型中发挥抗炎活性。我们试图探讨忍冬素在调节变应性气道炎症中的作用和机制。小鼠在出生后第5天(P5)和第10天腹腔注射卵清蛋白(OVA) 10 μg,然后在第18-20天每天吸入3%雾化OVA 10 min,建立哮喘小鼠模型。小鼠P16-P20灌胃Lonicerin(10或30 mg/kg)。值得注意的是,给药lonicerin可以改善炎症细胞的浸润和粘液的高分泌。治疗后,哮喘小鼠ova特异性IgE水平、炎症细胞计数和炎性细胞因子均明显降低。此外,通过降低赖氨酸27处组蛋白H3的三甲基化和减少NF-κB p65的核易位,可以抑制EZH2的活性和核因子κB (NF-ĸB)的活化。总之,忍冬素可能通过抑制EZH2/NF-κB信号通路减轻哮喘。
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Lonicerin alleviates ovalbumin-induced asthma of mice via inhibiting enhancer of zeste homolog 2/nuclear factor-kappa B signaling pathway.

Asthma is the most common chronic disease in the respiratory system of children caused by abnormal immunity that responses to common antigens. Lonicerin exerts anti-inflammatory activity in other inflammatory models through targeting enhancer of zeste homolog 2 (EZH2) that is related to asthma. We sought to explore the role and mechanism of lonicerin in regulating allergic airway inflammation. Mice were intraperitoneally injected 10 µg ovalbumin (OVA) on postnatal day 5 (P5) and P10, and then inhaled 3% aerosolized OVA for 10 min every day on P18-20, to establish asthmatic mice model. Lonicerin (10 or 30 mg/kg) was given to mice by intragastric administration on P16-P20. Notably, the administration of lonicerin amended infiltration of inflammatory cells and mucus hypersecretion. OVA-specific IgE level, inflammatory cell count and inflammatory cytokines in asthmatic mice were reduced after lonicerin treatment. Moreover, it suppressed the activity of EZH2 and activation of nuclear factor-kappa B (NF-κB) as evidenced by decreasing tri-methylation of histone H3 at lysine 27 and reducing nuclear translocation of NF-κB p65. In a word, Lonicerin may attenuate asthma by inhibiting EZH2/NF-κB signaling pathway.

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来源期刊
Experimental Animals
Experimental Animals 生物-动物学
CiteScore
2.80
自引率
4.20%
发文量
2
审稿时长
3 months
期刊介绍: The aim of this international journal is to accelerate progress in laboratory animal experimentation and disseminate relevant information in related areas through publication of peer reviewed Original papers and Review articles. The journal covers basic to applied biomedical research centering around use of experimental animals and also covers topics related to experimental animals such as technology, management, and animal welfare.
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