海报油墨组织标记在组织病理学中的比较效益

Dr. Ayesha Sarwar, Prof. Ashok Kumar Tanwni, Dr. Anum Usman, Khalida Moeed
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引用次数: 0

摘要

背景:用墨迹标记组织边缘在外科医生的决策中是重要的。本研究的目的是检查不同色调的当地可用的海报颜色在组织标记的可靠性,并评估颜色的可感知性在显微镜下与印度墨水标记的类似组织进行比较。方法:本实验研究于2021年2月27日至2021年4月29日在伊斯兰堡HBS医学与牙科学院和医院病理学系进行。样本量为50,采用方便抽样技术采集。选取五种福尔马林固定组织标本进行研究,以评价海报油墨在不同组织表面的标记效果。从每个标本的边缘处取4块切片。三种色调的海报颜色(黑色,蓝色和绿色)被用于墨水的三个部分,而每个组织类型的一个部分被标记为印度墨水。经过完整的组织处理和常规的苏木精和伊红(H&E)染色后,切片进行显微镜检查。评分是根据可见度从0到3分进行的。结果:本研究表明,与印度墨水相比,海报颜色墨水的组织是相当可靠的。与绿色相比,黑色和蓝色获得了最一致的结果。结论:在没有墨水的情况下,海报颜色是一种可靠的组织标记工具。它们有多种颜色可供选择,这使它们比印度墨水更有优势。关键词:伊红,印墨,切缘
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Comparative Benefits of Tissue Marking by Poster Ink in Histopathology
Background: Tissue margin marking with India ink is important in decision making for surgeons. The present study was conducted to examine the reliability of different shades of locally available poster colours in tissue marking and to evaluate the colour perceptibility microscopically in comparison with similar tissues marked by India ink. Methodology: This experimental study was conducted at Department of Pathology, HBS Medical & Dental College & Hospital, Islamabad from 27th February 2021 to 29th April 2021. Sample size was fifty, collected through convenient sampling technique. Five types of formalin fixed tissue specimens were selected for the study so as to evaluate the effectiveness of poster ink marking on different tissue surfaces. From each specimen, four sections were taken from the margins. Three shades of poster colour (black, blue and green) were used to ink three sections while one section from each tissue type was marked with India ink. After complete tissue processing and routine haematoxylin and eosin (H&E) staining, slides were examined microscopically. Scoring was done on a scale 0 to 3 on the basis of visibility. Results: The present study showed that poster colours inking of the tissues was quite reliable as compared to India ink. Most consistent results were achieved with black and blue colours as compared to green colour. Conclusion: Poster colours are reliable tool for tissue marking when India ink is not available. Their availability in a variety of colours provides them an edge over India ink. Key words: Eosin, India ink, Resection Margins
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