黄单胞菌AK几丁质酶ChiA基因的克隆、序列分析及ChiA的一些特性

Kazuo Sakka , Ryo Kusaka , Akihiro Kawano , Shuichi Karita , Jiraporn Sukhumavasi , Tetsuya Kimura , Kunio Ohmiya
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引用次数: 19

摘要

将编码几丁质酶A的chiA基因克隆到大肠杆菌中,并测定了其核苷酸序列。该结构基因全长1788 bp,编码596个氨基酸,预测分子量为62,122。推导出的ChiA是一种模块化酶,由一个n端信号肽和四个结构域组成,依次为:一个几丁质结合结构域、两个纤维连接蛋白III型结构域和一个家族18催化结构域。重组大肠杆菌纯化的ChiA的最适温度为35℃,pH为4.5℃。胶体甲壳素的Km和Vmax分别为1.8 mg/ml和8.7 μmol/min/mg。
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Cloning and sequencing of the gene encoding chitinase ChiA from Xanthomonas sp. strain AK and some properties of ChiA

The chiA gene encoding chitinase A was cloned into Escherichia coli from Xanthomonas sp. strain AK and its nucleotide sequence was determined. The structural gene consists of 1788 bp encoding 596 amino acids with a predicted molecular weight of 62,122. The deduced ChiA is a modular enzyme composed of an N-terminal signal peptide and four domains in the following order: a chitin-binding domain, two fibronectin type III domains, and a family 18 catalytic domain. ChiA purified from the recombinant E. coli had temperature and pH optima at 35°C and 4.5, respectively. The Km and Vmax values for colloidal chitin were estimated to be 1.8 mg/ml and 8.7 μmol/min/mg, respectively.

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