几种精油纳米乳液对草莓采后灰霉病的防治效果

E. El-argawy, Salma El-Dash, Abeer El-Ghannam, M. Ghozlan
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摘要

精油纳米乳剂在防治植物病原菌和提高植物抗微生物性能方面具有广阔的应用前景。本研究制备了丁香、玫瑰和佛手柑的纳米乳液精油,并测试了不同浓度的活性成分作为潜在的抗真菌剂对葡萄灰霉病(Botrytis cinerea isolate, BC4)的生长和对草莓采后灰霉病的控制。结果表明,丁香纳米乳剂在低浓度(80 μ ll -1)和低浓度(50 μ ll -1)下均能完全抑制灰绿芽孢杆菌(BC4)的生长和产孢。两种浓度(100µll -1和80µll -1)的丁香和玫瑰NEO在冷藏后7天完全抑制了灰霉病的发病率,而佛手柑NEO的效果一直较低。总的来说,与只接种分离真菌作为对照的草莓果实相比,接种分离真菌(BC4)的草莓果实在三个冷藏期的物理和化学特性,即水分含量、重量减轻、可滴定酸度和抗坏血酸含量显著保持在纳米精油(NEOs)处理下。关于水分
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EFFECACY OF SOME NANOEMULSSIONS OF ESSENTIAL OILS TO CONTROL STRAWBERRY GREY MOLD POST HARVEST DISEASE
Nano - emulsions of essential oils (NEOs) are a promising application in controlling plant pathogens, it also increases the antimicrobial properties. In this research, nano-emulsion essential oils of Clove, Rose and Bergamot were prepared and tested as potential antifungal agent at various concentrations of active ingredient against the growth of Botrytis cinerea isolate (BC4) and to control strawberry grey mold as post-harvest disease. Results indicated that Clove nano-emulsion was the most effective to completely inhibited the growth and sporulation of B. cinerea isolate, (BC4) at even the low concentration of 80 µll -1 for the growth and at concentration of at 50 µll -1 for sporulation tested fungus. Clove and Rose NEOs at both concentrations (100 µll -1 and 80 µll - 1) completely inhibited grey mold disease incidence at 7 days after fridge storage, However, Bergamot NEO consistently showed lower effect. In general, treatments with the tested nano-essential oils (NEOs) significantly maintained physical and chemical characteristics, i.e ., moisture content, weight loss, titrable acidity and ascorbic acid content of the treated strawberry fruits which inoculated with B. cinerea isolate (BC4), at the three periods of the fridge storage compared to the only inoculated fruits with fungus isolate as the control. Regarding the moisture
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