{"title":"寄生板栗培养物表达的溶胸酶、纤维素酶和蛋白酶活性,以及板栗和美洲板栗内皮提取物对这些活性的抑制作用","authors":"David R. McCarroll , Eyvind Thor","doi":"10.1016/0048-4059(85)90011-6","DOIUrl":null,"url":null,"abstract":"<div><p>Three classes of enzymatic activity were expressed in cultures of virulent and hypovirulent strains of <em>Endothia parasitica</em>. Polygalacturonase, cellulase, and protease activities accumulated in liquid cultures. Polygalacturonase activity was also detected in samples from active cankers resulting from infection by virulent strains of <em>E. parasitica</em>. The acidification of liquid media, as well as chestnut tissue in advance of growing mycelium, was determined and compared with the pH optima for the three enzyme activities. Additionally, polyphenolic extracts from <em>Castanea mollissima</em> were found to be more effective in eliminating polygalacturonase activity than were extracts from <em>Castanea dentata</em>. A specific inhibitor of polygalacturonase activity was also isolated. Polygalacturonase inhibitory activity extracted from <em>C. mollissima</em> inner bark was found to be more than twice as potent as the same activity extracted from <em>C. dentata</em> inner bark.</p></div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"26 3","pages":"Pages 367-378"},"PeriodicalIF":0.0000,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90011-6","citationCount":"26","resultStr":"{\"title\":\"Pectolytic, cellulytic and proteolytic activities expressed by cultures of Endothia parasitica, and inhibition of these activities by components extracted from Chinese and American chestnut inner bark\",\"authors\":\"David R. McCarroll , Eyvind Thor\",\"doi\":\"10.1016/0048-4059(85)90011-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Three classes of enzymatic activity were expressed in cultures of virulent and hypovirulent strains of <em>Endothia parasitica</em>. Polygalacturonase, cellulase, and protease activities accumulated in liquid cultures. Polygalacturonase activity was also detected in samples from active cankers resulting from infection by virulent strains of <em>E. parasitica</em>. The acidification of liquid media, as well as chestnut tissue in advance of growing mycelium, was determined and compared with the pH optima for the three enzyme activities. Additionally, polyphenolic extracts from <em>Castanea mollissima</em> were found to be more effective in eliminating polygalacturonase activity than were extracts from <em>Castanea dentata</em>. A specific inhibitor of polygalacturonase activity was also isolated. Polygalacturonase inhibitory activity extracted from <em>C. mollissima</em> inner bark was found to be more than twice as potent as the same activity extracted from <em>C. dentata</em> inner bark.</p></div>\",\"PeriodicalId\":101028,\"journal\":{\"name\":\"Physiological Plant Pathology\",\"volume\":\"26 3\",\"pages\":\"Pages 367-378\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1985-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0048-4059(85)90011-6\",\"citationCount\":\"26\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiological Plant Pathology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0048405985900116\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiological Plant Pathology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0048405985900116","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Pectolytic, cellulytic and proteolytic activities expressed by cultures of Endothia parasitica, and inhibition of these activities by components extracted from Chinese and American chestnut inner bark
Three classes of enzymatic activity were expressed in cultures of virulent and hypovirulent strains of Endothia parasitica. Polygalacturonase, cellulase, and protease activities accumulated in liquid cultures. Polygalacturonase activity was also detected in samples from active cankers resulting from infection by virulent strains of E. parasitica. The acidification of liquid media, as well as chestnut tissue in advance of growing mycelium, was determined and compared with the pH optima for the three enzyme activities. Additionally, polyphenolic extracts from Castanea mollissima were found to be more effective in eliminating polygalacturonase activity than were extracts from Castanea dentata. A specific inhibitor of polygalacturonase activity was also isolated. Polygalacturonase inhibitory activity extracted from C. mollissima inner bark was found to be more than twice as potent as the same activity extracted from C. dentata inner bark.