Mariyam Zuberi , Rashid Mir , Sagar Dholariya , Imtiyaz Najar , Prasant Yadav , Jamsheed Javid , Sameer Guru , Masroor Mirza , Gauri Gandhi , Nita Khurana , Prakash C. Ray , Alpana Saxena
{"title":"RASSF1和PTEN启动子超甲基化影响上皮性卵巢癌的预后","authors":"Mariyam Zuberi , Rashid Mir , Sagar Dholariya , Imtiyaz Najar , Prasant Yadav , Jamsheed Javid , Sameer Guru , Masroor Mirza , Gauri Gandhi , Nita Khurana , Prakash C. Ray , Alpana Saxena","doi":"10.1016/j.cogc.2014.12.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>To investigate the methylation pattern in promoter region of <em>RASSF1A</em> and <em>PTEN</em> genes in epithelial ovarian cancer patients in North India.</p></div><div><h3>Patients and Methods</h3><p>Fifty patients and 20 healthy controls were studied. Isolation of genomic DNA from peripheral blood and methylation-specific polymerase chain reaction (MSP) were applied for analysis.</p></div><div><h3>Results</h3><p>17 of 50 patients (34.0%) were found to be methylated for <em>RASSF1A</em> gene, whereas methylation of the <em>PTEN</em> gene occurred in 8 of 50 cases (16.0%). A statistically significant result was obtained (<em>P</em> = .01) for <em>RASSF1A</em> gene and correlated with the patients' clinicopathologic features.</p></div><div><h3>Conclusion</h3><p>Hypermethylation of both <em>RASSF1A</em> and <em>PTEN</em> genes in blood DNA from ovarian cancer patients might offer an exposition for early diagnosis of the malignancy.</p></div>","PeriodicalId":100274,"journal":{"name":"Clinical Ovarian and Other Gynecologic Cancer","volume":"7 1","pages":"Pages 33-39"},"PeriodicalIF":0.0000,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cogc.2014.12.002","citationCount":"11","resultStr":"{\"title\":\"RASSF1 and PTEN Promoter Hypermethylation Influences the Outcome in Epithelial Ovarian Cancer\",\"authors\":\"Mariyam Zuberi , Rashid Mir , Sagar Dholariya , Imtiyaz Najar , Prasant Yadav , Jamsheed Javid , Sameer Guru , Masroor Mirza , Gauri Gandhi , Nita Khurana , Prakash C. Ray , Alpana Saxena\",\"doi\":\"10.1016/j.cogc.2014.12.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>To investigate the methylation pattern in promoter region of <em>RASSF1A</em> and <em>PTEN</em> genes in epithelial ovarian cancer patients in North India.</p></div><div><h3>Patients and Methods</h3><p>Fifty patients and 20 healthy controls were studied. Isolation of genomic DNA from peripheral blood and methylation-specific polymerase chain reaction (MSP) were applied for analysis.</p></div><div><h3>Results</h3><p>17 of 50 patients (34.0%) were found to be methylated for <em>RASSF1A</em> gene, whereas methylation of the <em>PTEN</em> gene occurred in 8 of 50 cases (16.0%). A statistically significant result was obtained (<em>P</em> = .01) for <em>RASSF1A</em> gene and correlated with the patients' clinicopathologic features.</p></div><div><h3>Conclusion</h3><p>Hypermethylation of both <em>RASSF1A</em> and <em>PTEN</em> genes in blood DNA from ovarian cancer patients might offer an exposition for early diagnosis of the malignancy.</p></div>\",\"PeriodicalId\":100274,\"journal\":{\"name\":\"Clinical Ovarian and Other Gynecologic Cancer\",\"volume\":\"7 1\",\"pages\":\"Pages 33-39\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.cogc.2014.12.002\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Ovarian and Other Gynecologic Cancer\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212955315000022\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Ovarian and Other Gynecologic Cancer","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212955315000022","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
RASSF1 and PTEN Promoter Hypermethylation Influences the Outcome in Epithelial Ovarian Cancer
Background
To investigate the methylation pattern in promoter region of RASSF1A and PTEN genes in epithelial ovarian cancer patients in North India.
Patients and Methods
Fifty patients and 20 healthy controls were studied. Isolation of genomic DNA from peripheral blood and methylation-specific polymerase chain reaction (MSP) were applied for analysis.
Results
17 of 50 patients (34.0%) were found to be methylated for RASSF1A gene, whereas methylation of the PTEN gene occurred in 8 of 50 cases (16.0%). A statistically significant result was obtained (P = .01) for RASSF1A gene and correlated with the patients' clinicopathologic features.
Conclusion
Hypermethylation of both RASSF1A and PTEN genes in blood DNA from ovarian cancer patients might offer an exposition for early diagnosis of the malignancy.
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