两种杨树DNA胞嘧啶甲基化的全基因组分析

D. Liang, P. Shuai, X. Xia, W. Yin
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引用次数: 0

摘要

DNA甲基化是一种重要的表观遗传修饰,与许多生物过程有关。甲基化在应激反应和发育过程中的变化已经得到了很好的研究。在这里,我们提出了一个新的比较甲基化模式在两种杨树。利用甲基化DNA免疫沉淀测序技术(methylated DNA immune precipitation sequencing, MeDIPseq)检测了毛杨(Populus trichocarpa)和胡杨(Populus euphratica)叶片的甲基化模式。在两个物种中共检测到11个重要模块。我们的研究结果表明,毛藻中CG/CHH/CHG环境的甲基化要比胡杨显著得多,但在盐水胁迫下甲基化的变化在两种物种中是一致的。
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Genome-Wide Analysis of DNA Cytosine Methylation in Two Poplars
DNA methylation is an important epigenetic modification that has been implicated in many biological processes. Methylation changes during stress responses and developmental processes have been well studied. Here, we present a novel comparison of methylation patterns in two poplar species. We detected the global methylation patterns in leaves of Populus trichocarpa and Populus euphratica by methylated DNA immune precipitation sequencing (MeDIPseq). A total of eleven significant modules were detected in the two species. Our results indicate that the methylation of CG/CHH/CHG contexts was much more significant in P. trichocarpa than in P. euphratica, but methylation changes in response to saline stress were consistent in both species.
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