壳聚糖介导的银纳米粒子(Ag NPs)的合成及其抗菌应用前景

R. Kalaivani , M. Maruthupandy , T. Muneeswaran , A. Hameedha Beevi , M. Anand , C.M. Ramakritinan , A.K. Kumaraguru
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引用次数: 207

摘要

利用紫外可见光谱、傅里叶变换红外光谱、x射线衍射和透射电镜对壳聚糖介导的银纳米粒子(Ag NPs)进行了合成和表征。紫外可见吸收光谱显示,壳聚糖的加入提高了银纳米粒子的形成效率。此外,在较高的壳聚糖浓度下,NPs的尺寸减小更为显著。XRD图谱表明,Ag NPs为球形结晶结构(JCPDS卡号:65-2871)。原子力显微镜(AFM)和透射电镜(TEM)观察了合成的纳米粒子的表面形貌和尺寸。DLS图像证实了Ag NPs的大小分布和范围。合成的Ag NPs具有很强的抗菌和抗真菌活性。该研究表明,甲壳类动物的废物可能是生产银纳米粒子的一种更便宜的来源,银纳米粒子可用于生物医学研究的各种医学应用,并可能为海鲜产品(如几丁质和壳聚糖)开辟一个新的市场。
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Synthesis of chitosan mediated silver nanoparticles (Ag NPs) for potential antimicrobial applications

Synthesis of chitosan mediated silver nanoparticles (Ag NPs) and its characterization were fulfilled by UV–vis spectroscopy, fourier transform infrared spectroscopy, X-ray diffraction and transmission electron microscopy. UV–visible absorption spectrum revealed that the formation efficiency of Ag NPs was increased by the addition of chitosan. In addition, the size decrement of NPs was more remarkable at a higher chitosan concentration. XRD pattern has indicated that Ag NPs were spherical structured and crystalline in nature (JCPDS card no: 65-2871). The AFM and TEM images examined the surface morphology and the size of the synthesized NPs. DLS image confirmed the size distribution and range of the Ag NPs. The synthesized Ag NPs showed highly potent antibacterial and antifungal activities. The study suggested that crustacean waste could be a cheaper source for the production of Ag NPs which could be useful in various medical applications for biomedicine research and may create a new market for sea food with products such as chitin and chitosan.

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