香蕉CCO家族在发育、成熟和胁迫响应中的鉴定与表达

IF 1.3 4区 生物学 Q4 GENETICS & HEREDITY Genetica Pub Date : 2023-04-01 DOI:10.1007/s10709-023-00178-w
Liming Zeng, Liwang Zeng, Yu Wang, Zhengnan Xie, Minhua Zhao, Jie Chen, Xiaoxue Ye, Weiwei Tie, Meiying Li, Sang Shang, Libo Tian, Jian Zeng, Wei Hu
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引用次数: 0

摘要

植物激素脱落酸(ABA)在植物生长发育和对生物/非生物胁迫的响应中起着重要作用。因此,有必要研究与ABA合成相关的关键基因。目前,香蕉中作为ABA合成关键步骤的类胡萝卜素裂解加氧酶(CCOs)家族尚不清楚。本研究从香蕉基因组中鉴定出13个MaCCO基因和12个MbCCO基因,并将其分为NCED亚群和CCD亚群,确定了它们的进化关系、蛋白基序和基因结构。转录组学分析表明,CCO基因参与香蕉的发育、成熟以及对非生物和生物胁迫的响应,同源基因对在A或B亚基因组中表现出同源表达偏倚。结果表明,MaNCED3A、MaCCD1和MbNCED3B是果实发育和成熟过程中表达量最高的基因。MaNCED5 / MbNCED5和MaNCED9A可能对非生物胁迫有应答,MaNCED3A、3B、6a、9a和MbNCED9A的转录变化可能是对Foc4感染的应答。这些发现可能有助于确定ABA生物合成的关键酶,以及确定香蕉遗传改良的潜在靶点。
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Identification and expression of the CCO family during development, ripening and stress response in banana.

Plant hormone abscisic acid (ABA) plays an important role in plant growth, development and response to biotic / abiotic stressors. Thus, it is necessary to investigate the crucial genes associated with ABA synthesis. Currently, the carotenoid cleavage oxygenases (CCOs) family that function as the key step for ABA synthesis are not well understood in banana. In this study, 13 MaCCO genes and 12 MbCCO genes, divided into NCED subgroup and CCD subgroup, were identified from the banana genome, and their evolutionary relationship, protein motifs, and gene structures were also determined. Transcriptomic analysis suggested the involvement of CCO genes in banana development, ripening, and response to abiotic and biotic stressors, and homologous gene pairs showed homoeologue expression bias in the A or B subgenome. Our results identified MaNCED3A, MaCCD1, and MbNCED3B as the genes with the highest expression during fruit development and ripening. MaNCED5 / MbNCED5 and MaNCED9A might respond to abiotic stress, and MaNCED3A, 3B, 6 A, 9 A, and MbNCED9A showed transcriptional changes that could be a response to Foc4 infection. These findings may contribute to the characterization of key enzymes involved in ABA biosynthesis, as well as to identify potential targets for the genetic improvement of banana.

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来源期刊
Genetica
Genetica 生物-遗传学
CiteScore
2.70
自引率
0.00%
发文量
32
审稿时长
>12 weeks
期刊介绍: Genetica publishes papers dealing with genetics, genomics, and evolution. Our journal covers novel advances in the fields of genomics, conservation genetics, genotype-phenotype interactions, evo-devo, population and quantitative genetics, and biodiversity. Genetica publishes original research articles addressing novel conceptual, experimental, and theoretical issues in these areas, whatever the taxon considered. Biomedical papers and papers on breeding animal and plant genetics are not within the scope of Genetica, unless framed in an evolutionary context. Recent advances in genetics, genomics and evolution are also published in thematic issues and synthesis papers published by experts in the field.
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