转录因子 Foxi1 可促进 M-1 细胞中 V-ATPase 和 Gpr116 的表达。

IF 3.7 2区 医学 Q1 PHYSIOLOGY American Journal of Physiology-renal Physiology Pub Date : 2023-03-01 Epub Date: 2023-01-05 DOI:10.1152/ajprenal.00272.2022
Mackenzie Kui, Jennifer L Pluznick, Nathan A Zaidman
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引用次数: 0

摘要

每个肾小管节段的不同功能都有赖于特化细胞群的协调作用,而特化细胞群的转录特征对其有独特的定义。在集合管中,有两个关键而独特的细胞群:主细胞和夹层细胞。主细胞在水、Na+ 和 K+ 的调节中发挥关键作用,而闰层细胞在酸碱平衡中的作用最为人熟知。目前,还没有能再现集合管异质性的体外系统,这限制了对遗传和生理现象的高通量和重复性研究。在这里,我们证明了转录因子 Foxi1 足以改变小鼠皮质集合管细胞系 M-1 细胞的转录特性。具体来说,过表达 Foxi1 会诱导闰细胞转录本的表达,包括 Gpr116、Atp6v1b1、Atp6v1g3、Atp6v0d2、Slc4a9 和 Slc26a4。这些数据表明,过表达 Foxi1 能使 M-1 细胞向非 A、非 B 型闰层细胞表型分化,并为研究肾集合管的转录调控和生理功能提供了一种新型体外工具。这种简单而新颖的体外系统可用于研究肾闰细胞的转录调控、细胞规格和分化等过程。
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The transcription factor Foxi1 promotes expression of V-ATPase and Gpr116 in M-1 cells.

The diverse functions of each nephron segment rely on the coordinated action of specialized cell populations that are uniquely defined by their transcriptional profile. In the collecting duct, there are two critical and distinct cell populations: principal cells and intercalated cells. Principal cells play key roles in the regulation of water, Na+, and K+, whereas intercalated cells are best known for their role in acid-base homeostasis. Currently, there are no in vitro systems that recapitulate the heterogeneity of the collecting ducts, which limits high-throughput and replicate investigations of genetic and physiological phenomena. Here, we demonstrated that the transcription factor Foxi1 is sufficient to alter the transcriptional identity of M-1 cells, a murine cortical collecting duct cell line. Specifically, overexpression of Foxi1 induces the expression of intercalated cell transcripts including Gpr116, Atp6v1b1, Atp6v1g3, Atp6v0d2, Slc4a9, and Slc26a4. These data indicate that overexpression of Foxi1 differentiates M-1 cells toward a non-A, non-B type intercalated cell phenotype and may provide a novel in vitro tool to study transcriptional regulation and physiological function of the renal collecting duct.NEW & NOTEWORTHY Transfection of M-1 cells with the transcription factor Foxi1 generates cells that express V-ATPase and Gpr116 as well as other genes associated with renal intercalated cells. This straightforward and novel in vitro system could be used to study processes including transcriptional regulation and cell specification and differentiation in renal intercalated cells.

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来源期刊
CiteScore
8.40
自引率
7.10%
发文量
154
审稿时长
2-4 weeks
期刊介绍: The American Journal of Physiology - Renal Physiology publishes original manuscripts on timely topics in both basic science and clinical research. Published articles address a broad range of subjects relating to the kidney and urinary tract, and may involve human or animal models, individual cell types, and isolated membrane systems. Also covered are the pathophysiological basis of renal disease processes, regulation of body fluids, and clinical research that provides mechanistic insights. Studies of renal function may be conducted using a wide range of approaches, such as biochemistry, immunology, genetics, mathematical modeling, molecular biology, as well as physiological and clinical methodologies.
期刊最新文献
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