运动训练后肌肉中lncRNA-miRNA-mRNA竞争内源性RNA网络的构建

IF 1.3 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY General physiology and biophysics Pub Date : 2023-03-01 DOI:10.4149/gpb_2022062
Mingkun Nie, Qingling Liu, Cheng Yan
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引用次数: 0

摘要

缺乏运动显然是许多疾病的危险因素,包括心血管疾病、糖尿病、癌症等。越来越多的证据表明,RNA作为竞争性内源性RNA (ceRNA),在运动训练后骨骼肌的适应性变化中起着重要作用。虽然运动诱导的健身对骨骼肌的影响已经很好地确立,但其潜在的机制尚未完全了解。本研究的目的是构建一个新的骨骼肌ceRNA网络,以响应运动训练。骨骼肌基因表达谱从GEO数据库下载。然后,我们鉴定了运动前和运动后样本之间差异表达的lncrna、mirna和mrna。随后,我们基于ceRNA理论构建了lncRNA-miRNA-mRNA调控网络。共鉴定出1153个mrna(687个上调,466个下调)、7个mirna(3个上调,4个下调)和5个lncRNAs(3个上调,2个下调)为差异表达基因。获得3个lncrna、5个mirna和227个mrna,构建mirna介导的ceRNA网络。我们在肌肉中构建了一个新的ceRNA调节网络,以响应运动训练,这为体育活动带来的健康益处的分子机制提供了见解。
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Construction of a novel lncRNA-miRNA-mRNA competing endogenous RNA network in muscle in response to exercise training.

Physical inactivity has evidently been a hazard factor for many diseases, including cardiovascular disease, diabetes, cancer, etc. Rising evidence indicates that RNA, as competitive endogenous RNA (ceRNA), plays an important role in adaptive changes in skeletal muscle in response to exercise training. Although the effects of exercise-induced fitness on skeletal muscle have been well established, the mechanisms underlying are not fully understood. The purpose of this study is to construct a novel ceRNA network in skeletal muscle in response to exercise training. Skeletal muscle gene expression profiles were downloaded from the GEO database. Then, we identified differentially expressed lncRNAs, miRNAs, and mRNAs between the pre-exercise and post-exercise samples. Subsequently, we constructed lncRNA-miRNA-mRNA regulatory networks based on the ceRNA theory. 1153 mRNAs (687 upregulated and 466 downregulated), 7 miRNAs (3 upregulated and 4 downregulated), and 5 lncRNAs (3 upregulated and 2 downregulated) were identified as differentially expressed genes. 3 lncRNAs, 5 miRNAs and 227 mRNAs were obtained to build miRNA-mediated ceRNA networks. We constructed a novel ceRNA regulatory network in muscle in response to exercise training, which provides insights into molecular mechanisms underlying the health benefits brought by physical activity.

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来源期刊
General physiology and biophysics
General physiology and biophysics 生物-生化与分子生物学
CiteScore
2.70
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: General Physiology and Biophysics is devoted to the publication of original research papers concerned with general physiology, biophysics and biochemistry at the cellular and molecular level and is published quarterly by the Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences.
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