高效液相色谱法测定新生儿血浆帕尼培南浓度。

H. Kokubun, Toshimi Kimura, S. Murase, S. Shimada, H. Kubo, M. Matsumoto, M. Nowatari, N. Matsuura
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引用次数: 1

摘要

建立了用反相高效液相色谱法快速测定极低出生体重儿血浆帕尼培南(PAPM)的方法。流动相为35%甲醇(pH为5.8),溶液为5mm NaH2PO4和5mm十二烷基硫酸钠。流速0.8 mL/min。在300 nm处进行紫外检测。预处理方法为:在血浆中加入200 mM 3[morpholino]丙磺酸溶液作为稳定剂。然后用甲醇使其脱蛋白。校准曲线在6.25 itƒÊg/mL ~ 100ƒÊg/mL范围内呈线性关系。已知浓度样品的回收率为98.4%。运行内和日常变化分别低于2.5%和2.7%。测定了5例婴儿血浆中PAPM的浓度。在未成熟的患者中,还观察到半衰期延长。
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Determination of Plasma Panipenem Concentration in Neonates by High-Performance Liquid Chromatography.
A simple and fast determination of plasma panipenem (PAPM) in extremely low birth weight infants was established using reversed phase high-performance liquid chromatography. Solutions of 35% methanol (pH 5.8), including 5 mM NaH2PO4 and 5 mM sodium dodecylsulfate, were used for the mobile phase. The flow rate was 0.8 mL/min. UV detection was carried out at 300 nm. The pretreatment method was as follows : 200 mM 3[morpholino] propansulfonic acid solution was added to the plasma as a stabilizer. It was then deproteinized with methanol. The calibration curve was linear in a range from 6.25 itƒÊg/mL to 100ƒÊg/mL. The recovery rate from known concentration samples of PAPM was 98.4%. The within-run and day-to-day variations were below 2.5% and 2.7%, respectively. The PAPM concentrations in the plasma were determined in five infants. In immature patients, an extension of the half-life was also observed.
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