胡萝卜胚性细胞、非胚性细胞和体胚中脱落酸内源水平的研究

Tomohiro Kiyosue , Masatoshi Nakajima , Isomaro Yamaguchi , Shinobu Satoh , Hiroshi Kamada , Hiroshi Harada
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引用次数: 32

摘要

采用ELISA法测定了胡萝卜胚性细胞、非胚性细胞和体胚中内源ABA的含量。据估计,胚性细胞的ABA含量比非胚性细胞高67.2倍,比体细胞胚胎高2.5倍。探讨了内源ABA水平与胡萝卜培养细胞胚发生能力的关系。
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Endogenous Levels of Abscisic Acid in Embryogenic Cells, Nonembryogenic Cells and Somatic Embryos of Carrot (Daucus carota L.)

The levels of endogenous ABA were measured by ELISA in embryogenic cells, nonembryogenic cells and somatic embryos of carrot. Embryogenic cells were estimated to contain a 67.2 times higher level of ABA than non-embryogenic cells, and a 2.5 times higher level than somatic embryos. The correlation between the endogenous level of ABA and embryogenic competence of carrot cultured cells is discussed.

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