{"title":"印度旁遮普邦班迪科塔孟加拉人聚居区巴西乳头状肌严重感染的流行率、危险因素分析、分子鉴定和系统发育","authors":"Shivani Rara, Neena Singla, Sukhmanpreet Kaur Brar, Dimple Mandla, Lachhman Das Singla","doi":"10.1007/s11686-022-00652-z","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>The present study aimed to record the prevalence, risk factors, molecular identification, and phylogeny of <i>Nippostrongylus brasiliensis</i> found in the small intestine of the lesser bandicoot rat, <i>Bandicota bengalensis,</i> a wild rodent species.</p><h3>Methods</h3><p>A total of 100 bandicoot rats live trapped at two commensal urban locations (50 each), i.e., a fish market and railway station, in Ludhiana, Punjab State (India), from November 2020 to October 2021, were analysed for the presence of <i>N. brasiliensis,</i> a nematode parasite of zoonotic importance.</p><h3>Result</h3><p>Overall, the small intestine of 43.00% of the rats was found severely infected with bright red coloured adult <i>N. brasiliensis</i> of both sexes (total of 1439 specimens). Faecal samples contained ellipsoidal and thin-shelled eggs measuring 62.25–74.70 m in length and 33.20–37.35 m in breadth. No significant (<i>P</i> > 0.05) effect of host age, sex, or season was observed on the rate of infection. The parasite intensity and mean abundance ranged from 27.68–38.04 and 10.52–18.26, respectively, indicating a high risk of disease transmission. Based on the morphology, the nematode parasite was identified as <i>Nippostrongylus</i> sp. Molecular identification was confirmed through PCR amplification of the mitochondrial cytochrome oxidase I gene, which showed a single band of approximately 355 bp. A comparison of the present isolate with the available sequences of <i>Nippostrongylus</i> species across the globe showed 100% nucleotide homology with <i>N. brasiliensis</i> sequences available in GenBank from Japan (AP017690), the USA (U57035), and New Zealand (NC033886).</p><h3>Conclusion</h3><p>The study indicates that <i>B. bengalensis</i> inhabiting commensal urban areas is a reservoir host for <i>N. brasiliensis,</i> which if transmitted to humans and animals visiting the area may pose a potential health risk. The study thus suggests proper rodent population management close to human habitations to avoid the transmission of disease-causing agents.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"68 1","pages":"172 - 181"},"PeriodicalIF":1.2000,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Severe Infection of Nippostrongylus brasiliensis in Bandicota bengalensis Inhabiting Commensal Areas of Punjab, India: Prevalence, Risk Factor Analysis, Molecular Identification and Phylogenesis\",\"authors\":\"Shivani Rara, Neena Singla, Sukhmanpreet Kaur Brar, Dimple Mandla, Lachhman Das Singla\",\"doi\":\"10.1007/s11686-022-00652-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>The present study aimed to record the prevalence, risk factors, molecular identification, and phylogeny of <i>Nippostrongylus brasiliensis</i> found in the small intestine of the lesser bandicoot rat, <i>Bandicota bengalensis,</i> a wild rodent species.</p><h3>Methods</h3><p>A total of 100 bandicoot rats live trapped at two commensal urban locations (50 each), i.e., a fish market and railway station, in Ludhiana, Punjab State (India), from November 2020 to October 2021, were analysed for the presence of <i>N. brasiliensis,</i> a nematode parasite of zoonotic importance.</p><h3>Result</h3><p>Overall, the small intestine of 43.00% of the rats was found severely infected with bright red coloured adult <i>N. brasiliensis</i> of both sexes (total of 1439 specimens). Faecal samples contained ellipsoidal and thin-shelled eggs measuring 62.25–74.70 m in length and 33.20–37.35 m in breadth. No significant (<i>P</i> > 0.05) effect of host age, sex, or season was observed on the rate of infection. The parasite intensity and mean abundance ranged from 27.68–38.04 and 10.52–18.26, respectively, indicating a high risk of disease transmission. Based on the morphology, the nematode parasite was identified as <i>Nippostrongylus</i> sp. Molecular identification was confirmed through PCR amplification of the mitochondrial cytochrome oxidase I gene, which showed a single band of approximately 355 bp. A comparison of the present isolate with the available sequences of <i>Nippostrongylus</i> species across the globe showed 100% nucleotide homology with <i>N. brasiliensis</i> sequences available in GenBank from Japan (AP017690), the USA (U57035), and New Zealand (NC033886).</p><h3>Conclusion</h3><p>The study indicates that <i>B. bengalensis</i> inhabiting commensal urban areas is a reservoir host for <i>N. brasiliensis,</i> which if transmitted to humans and animals visiting the area may pose a potential health risk. The study thus suggests proper rodent population management close to human habitations to avoid the transmission of disease-causing agents.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"68 1\",\"pages\":\"172 - 181\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2022-12-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-022-00652-z\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-022-00652-z","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Severe Infection of Nippostrongylus brasiliensis in Bandicota bengalensis Inhabiting Commensal Areas of Punjab, India: Prevalence, Risk Factor Analysis, Molecular Identification and Phylogenesis
Purpose
The present study aimed to record the prevalence, risk factors, molecular identification, and phylogeny of Nippostrongylus brasiliensis found in the small intestine of the lesser bandicoot rat, Bandicota bengalensis, a wild rodent species.
Methods
A total of 100 bandicoot rats live trapped at two commensal urban locations (50 each), i.e., a fish market and railway station, in Ludhiana, Punjab State (India), from November 2020 to October 2021, were analysed for the presence of N. brasiliensis, a nematode parasite of zoonotic importance.
Result
Overall, the small intestine of 43.00% of the rats was found severely infected with bright red coloured adult N. brasiliensis of both sexes (total of 1439 specimens). Faecal samples contained ellipsoidal and thin-shelled eggs measuring 62.25–74.70 m in length and 33.20–37.35 m in breadth. No significant (P > 0.05) effect of host age, sex, or season was observed on the rate of infection. The parasite intensity and mean abundance ranged from 27.68–38.04 and 10.52–18.26, respectively, indicating a high risk of disease transmission. Based on the morphology, the nematode parasite was identified as Nippostrongylus sp. Molecular identification was confirmed through PCR amplification of the mitochondrial cytochrome oxidase I gene, which showed a single band of approximately 355 bp. A comparison of the present isolate with the available sequences of Nippostrongylus species across the globe showed 100% nucleotide homology with N. brasiliensis sequences available in GenBank from Japan (AP017690), the USA (U57035), and New Zealand (NC033886).
Conclusion
The study indicates that B. bengalensis inhabiting commensal urban areas is a reservoir host for N. brasiliensis, which if transmitted to humans and animals visiting the area may pose a potential health risk. The study thus suggests proper rodent population management close to human habitations to avoid the transmission of disease-causing agents.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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