苍蝇发作脑电图:果蝇大脑的场电位活动。

IF 1.8 4区 医学 Q3 GENETICS & HEREDITY Journal of neurogenetics Pub Date : 2021-09-01 DOI:10.1080/01677063.2021.1950714
Atulya Iyengar, Chun-Fang Wu
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引用次数: 0

摘要

超同步神经活动是癫痫发作的一个特征。尽管许多癫痫相关基因的果蝇突变体表现出明显的行为痉挛和运动单位过度兴奋性,但癫痫发作期间大脑区域异常超同步活动的场电位测量尚未得到描述。在这里,我们报告了一种直接观察果蝇大脑局部场电位(LFPs)的方法,以监测行为系缚果蝇癫痫发作期间的整体神经活动。通过大脑的高频刺激可触发电惊厥发作(ECS)脉冲放电的刻板序列,这在背纵肌(DLM)中很容易检测到,并伴有行为痉挛。在癫痫发作期间,LFP信号表现出特征性的大振幅振荡,与DLM飞行肌尖峰具有典型的时间相关性。ecs相关的LFP事件明显不同于休息和飞行相关的LFP模式。我们进一步表征了LFP在不同类型的癫痫发作中由遗传和药理学操作引起的活性。在“bang-sensitive”钠通道突变体(bss)中,LFP模式被延长,LFP振荡与DLM放电之间的时间相关性被改变。在给予促惊厥GABAA阻滞剂微毒素后,我们发现LFP活性模式在质量上有所不同,该模式由缓慢(1 hz),重复的波形组成,与DLM破裂和行为痉挛密切相关。我们记录大脑lfp的方法为大量果蝇兴奋性突变体中复杂的全脑活动模式的电生理分析提供了一个初始框架。
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Fly seizure EEG: field potential activity in the Drosophila brain.

Hypersynchronous neural activity is a characteristic feature of seizures. Although many Drosophila mutants of epilepsy-related genes display clear behavioral spasms and motor unit hyperexcitability, field potential measurements of aberrant hypersynchronous activity across brain regions during seizures have yet to be described. Here, we report a straightforward method to observe local field potentials (LFPs) from the Drosophila brain to monitor ensemble neural activity during seizures in behaving tethered flies. High frequency stimulation across the brain reliably triggers a stereotypic sequence of electroconvulsive seizure (ECS) spike discharges readily detectable in the dorsal longitudinal muscle (DLM) and coupled with behavioral spasms. During seizure episodes, the LFP signal displayed characteristic large-amplitude oscillations with a stereotypic temporal correlation to DLM flight muscle spiking. ECS-related LFP events were clearly distinct from rest- and flight-associated LFP patterns. We further characterized the LFP activity during different types of seizures originating from genetic and pharmacological manipulations. In the 'bang-sensitive' sodium channel mutant bangsenseless (bss), the LFP pattern was prolonged, and the temporal correlation between LFP oscillations and DLM discharges was altered. Following administration of the pro-convulsant GABAA blocker picrotoxin, we uncovered a qualitatively different LFP activity pattern, which consisted of a slow (1-Hz), repetitive, waveform, closely coupled with DLM bursting and behavioral spasms. Our approach to record brain LFPs presents an initial framework for electrophysiological analysis of the complex brain-wide activity patterns in the large collection of Drosophila excitability mutants.

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来源期刊
Journal of neurogenetics
Journal of neurogenetics 医学-神经科学
CiteScore
4.40
自引率
0.00%
发文量
13
审稿时长
>12 weeks
期刊介绍: The Journal is appropriate for papers on behavioral, biochemical, or cellular aspects of neural function, plasticity, aging or disease. In addition to analyses in the traditional genetic-model organisms, C. elegans, Drosophila, mouse and the zebrafish, the Journal encourages submission of neurogenetic investigations performed in organisms not easily amenable to experimental genetics. Such investigations might, for instance, describe behavioral differences deriving from genetic variation within a species, or report human disease studies that provide exceptional insights into biological mechanisms
期刊最新文献
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