血浆和尿液中的长链非编码rna作为前列腺癌的潜在生物标志物

Ionuț Andrei Păunescu, R. Bardan, B. Petrut, O. Bălăcescu, A. Cumpănaş, A. Dema, A. Marcu, C. Marian, E. Seclaman, I. Sîrbu
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引用次数: 0

摘要

(1)引言:前列腺癌是发达国家男性癌症相关死亡的第二大原因。由于现有生物标志物的局限性,迫切需要开发新的、更好的非侵入性前列腺癌诊断和监测标志物。(2)材料与方法:采用real-time PCR技术检测84种长链非编码RNA (lncRNA)在前列腺癌患者血浆和尿液外泌体中的表达水平。(3)结果:在所有样品类型中,只有少数lncrna检测到高丰度(Ct在25到30个循环之间),绝大多数显示相对适度的水平(Ct在50到30个循环之间)。正如预期的那样,血浆和血浆外泌体比尿液含有更多的lncRNA种类,无论它们是来自患者还是对照组。我们在前列腺癌样本中发现了两种具有统计学意义的失调lncRNAs: RBM5-AS1在血浆中下调2.89倍(p = 0.036), SNHG16在尿外泌体中上调13.69倍(p = 0.029)。(4)结论:这些初步数据需要在其他独立的、更广泛的研究中进一步验证,才能被认为是前列腺癌的生物标志物。
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Long Non-Coding RNAs in Plasma and Urine as Potential Biomarkers in Prostate Cancer
(1) Introduction: Prostate cancer is the second leading cause of cancer-related death in men in developed countries. Due to the existing biomarkers’ limitations, there is a stringent need to develop novel, better non-invasive markers for prostate cancer diagnostic and monitoring. (2) Material and methods: We assessed, by real-time PCR, the expression level of 84 long non-coding RNA (lncRNA) in plasma and the exosomes isolated from prostate cancer patients’ plasma and urine. (3) Results: Only a few lncRNAs were detected in high abundance (Ct between 25 and 30 cycles) across all sample types, the vast majority showing relatively modest levels (Ct > 30 cycles). As expected, plasma and plasma exosomes contain far more lncRNA species than urine, irrespective of whether they originate from patients or controls. We identified two statistically significant dysregulated lncRNAs in prostate cancer samples vs. controls: RBM5-AS1, 2.89 times downregulated in plasma (p = 0.036), and SNHG16, 13.69 times upregulated (p = 0.029) in urine exosomes. (4) Conclusions: These preliminary data need further validation in additional independent, more extensive studies before they can be considered as biomarkers for prostate cancer.
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