考虑毛状根高密度培养过程中生物量变化的生物量估算程序

Kyung-Hee Jung, Sang-Soo Kwak, Jang R. Liu
{"title":"考虑毛状根高密度培养过程中生物量变化的生物量估算程序","authors":"Kyung-Hee Jung,&nbsp;Sang-Soo Kwak,&nbsp;Jang R. Liu","doi":"10.1016/S0922-338X(98)80095-0","DOIUrl":null,"url":null,"abstract":"<div><p>The biomass concentration in a bubble column reactor was estimated by analyzing the conductivity change during high-density culture of <em>Catharanthus roseus</em> hairy roots. The accumulated biomass in the reactor was estimated from the amount of nutrients consumed, which were present in the liquid medium. Liquid medium, consisting of water and nutrients, was incorporated into the biomass, in proportion to the growth of hairy roots, consequently the ratio of the volume of liquid medium to the volume of the biomass decreased continuously. To evaluate nutrient consumption, the change in biomass volume in the reactor was introduced as a new parameter for biomass estimation. After 45 d of culture, the final concentration of hairy roots was 46.5 g dry wt./<em>l</em>, of which the biomass volume is about 40% of the total culture volume of the reactor. A conventional conductivity method, in which the volume change of the liquid medium was not considered, resulted in a deviation (55.76 g dry wt./<em>l</em>) of about 20% from the actual biomass. This procedure yielded a value of 47.1 g dry wt./<em>l</em> of hairy roots, which is a nearly accurate estimation of the biomass concentration. This result suggests that the volume reduction of liquid medium should be considered for biomass estimation and process control for high-density plant cell cultures.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 4","pages":"Pages 454-457"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80095-0","citationCount":"7","resultStr":"{\"title\":\"Procedure for biomass estimation considering the change in biomass volume during high density culture of hairy roots\",\"authors\":\"Kyung-Hee Jung,&nbsp;Sang-Soo Kwak,&nbsp;Jang R. Liu\",\"doi\":\"10.1016/S0922-338X(98)80095-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The biomass concentration in a bubble column reactor was estimated by analyzing the conductivity change during high-density culture of <em>Catharanthus roseus</em> hairy roots. The accumulated biomass in the reactor was estimated from the amount of nutrients consumed, which were present in the liquid medium. Liquid medium, consisting of water and nutrients, was incorporated into the biomass, in proportion to the growth of hairy roots, consequently the ratio of the volume of liquid medium to the volume of the biomass decreased continuously. To evaluate nutrient consumption, the change in biomass volume in the reactor was introduced as a new parameter for biomass estimation. After 45 d of culture, the final concentration of hairy roots was 46.5 g dry wt./<em>l</em>, of which the biomass volume is about 40% of the total culture volume of the reactor. A conventional conductivity method, in which the volume change of the liquid medium was not considered, resulted in a deviation (55.76 g dry wt./<em>l</em>) of about 20% from the actual biomass. This procedure yielded a value of 47.1 g dry wt./<em>l</em> of hairy roots, which is a nearly accurate estimation of the biomass concentration. This result suggests that the volume reduction of liquid medium should be considered for biomass estimation and process control for high-density plant cell cultures.</p></div>\",\"PeriodicalId\":15696,\"journal\":{\"name\":\"Journal of Fermentation and Bioengineering\",\"volume\":\"85 4\",\"pages\":\"Pages 454-457\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80095-0\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fermentation and Bioengineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0922338X98800950\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation and Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0922338X98800950","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7

摘要

通过分析花楸毛状根高密度培养过程中电导率的变化,估算了泡塔反应器中生物量的浓度。反应器中积累的生物量是根据存在于液体介质中的营养物质的消耗量来估计的。在生物量中加入由水和营养物质组成的液体培养基,与毛状根的生长成比例,因此液体培养基的体积与生物量的体积之比不断减小。为了评估养分消耗,将反应器内生物量的变化作为估算生物量的新参数。培养45 d后,毛状根终浓度为46.5 g干wt./l,其生物量约占反应器总培养体积的40%。传统的电导率法,在不考虑液体介质体积变化的情况下,与实际生物量的偏差(55.76 g干wt./l)约为20%。这个过程产生了47.1 g干重/升毛状根的值,这是一个几乎准确的生物量浓度估计。这一结果表明,在高密度植物细胞培养过程中,应考虑减少液体培养基的体积来估算生物量和控制过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Procedure for biomass estimation considering the change in biomass volume during high density culture of hairy roots

The biomass concentration in a bubble column reactor was estimated by analyzing the conductivity change during high-density culture of Catharanthus roseus hairy roots. The accumulated biomass in the reactor was estimated from the amount of nutrients consumed, which were present in the liquid medium. Liquid medium, consisting of water and nutrients, was incorporated into the biomass, in proportion to the growth of hairy roots, consequently the ratio of the volume of liquid medium to the volume of the biomass decreased continuously. To evaluate nutrient consumption, the change in biomass volume in the reactor was introduced as a new parameter for biomass estimation. After 45 d of culture, the final concentration of hairy roots was 46.5 g dry wt./l, of which the biomass volume is about 40% of the total culture volume of the reactor. A conventional conductivity method, in which the volume change of the liquid medium was not considered, resulted in a deviation (55.76 g dry wt./l) of about 20% from the actual biomass. This procedure yielded a value of 47.1 g dry wt./l of hairy roots, which is a nearly accurate estimation of the biomass concentration. This result suggests that the volume reduction of liquid medium should be considered for biomass estimation and process control for high-density plant cell cultures.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Author index Author index Author index Keyword Index Author Index
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1