尼日利亚河流州Elele社区当地消费棕榈酒的微生物质量分析

I. Ikeh, B. C. Anele, C. C. Ukanwa, S. Njoku
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摘要

由于棕榈酒对人体系统的营养成分,特别是新鲜和未发酵的状态,棕榈酒通常被消费。从河流州Elele社区的两个不同地点获得的总共20份棕榈酒样品进行了微生物质量分析。采用10倍连续稀释法。总需氧平板计数(TAPC)采用营养琼脂,大肠菌群计数(CC)采用麦康基(MacConkey),大肠杆菌计数(EC)采用伊红亚甲基蓝,真菌计数采用马铃薯葡萄糖琼脂。在酒吧销售的棕榈酒中微生物计数高于棕榈酒tapper。饮用棒样品的TAPC平均值(6.73+ 0.22 log10cfu/ml)高于棕榈酒tapper的平均值(6.70+0.15log10cfu/ml)。饮条棕榈酒的大肠菌群数量为(6.57+ 0.10log10cfu/ml),与从tapper中获得的最低数量(6.56+ 0.9log10cfu/ml)差异不显著。酒吧棕榈酒的大肠杆菌含量为(5.73+ 0.23 log10cfu/ml)高于(5.71+ 0.18 log10cfu/ml)。从酒吧间取样的棕榈酒的真菌计数较高,但与从tapper取样的没有显著差异。从两种棕榈酒中分离出的细菌分别为葡萄球菌50%和30%,克雷伯氏菌20%和30%,变形杆菌40%和10%和30%,曲霉30%和10%,酿酒酵母20%和30%。对于方差分析,细菌和真菌数量不显著。建议棕榈酒的消费者从饮酒者处购买产品,以减少受污染的机会。
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Analysis of the Microbial Quality of Locally Consumed Palm Wine Sold in Elele Community of Rivers State Nigeria
Palm wine is generally consumed due to its nutritive composition to the human body system particularly when fresh and unfermented state. A total of 20 Palm wine samples obtained from two different locations in Elele community of Rivers state, were analyzed for their microbiological qualities. A ten-fold serial dilution method was used. For Total Aerobic Plate Count (TAPC) nutrient agar was used, MacConkey for coliform count (CC), Eosin methylene blue for Escherichia coli count (EC), and Potato dextrose agar for the fungal count. Microbial counts in the palm wine sold in the drinking bar were higher than that of the palm wine tapper.  TAPC, the sample from the drinking bar has a mean value (6.73+ 0.22 log10cfu/ml) which was higher than the value obtained from the palm wine tapper (6.70+0.15log10cfu/ml). The coliform count of palm wine from the drinking bar was (6.57+ 0.10log10cfu/ml) but not significantly different from those with minimum counts (6.56+ 0.9log10cfu/ml) obtained from the tapper. Escherichia coli of palm wine from drinking bar were (5.73+ 0.23 log10cfu/ml) which were higher than (5.71+ 0.18 log10cfu/ml). The Fungal counts of palm wine sampled from the drinking bar were higher but not significantly different from those obtained from the tapper. Bacteria isolated from the two respective palm wines sampled included Staphylococcus spp 50% and 30% respectively, Klebsiella spp 20% and 30% respectively, Proteus spp 40% and 10% and 30% respectively, Aspergillus spp 30% ,  10% and Saccharomyce cerevisae 20% and 30% respectively. For the analysis of variance, bacteria and fungi count was not significant. Consumers of palm wine are advised to purchase the product from the tapper to reduce the chances of contamination.
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