D-[1-14C]半乳糖胺在大鼠血清蛋白和组织中的掺入

Betty N. White , M.R. Shetlar , H.M. Shurley , J.A. Schilling
{"title":"D-[1-14C]半乳糖胺在大鼠血清蛋白和组织中的掺入","authors":"Betty N. White ,&nbsp;M.R. Shetlar ,&nbsp;H.M. Shurley ,&nbsp;J.A. Schilling","doi":"10.1016/0926-6534(65)90003-5","DOIUrl":null,"url":null,"abstract":"<div><p>Incorporation of <span>D</span>-[1-<sup>14</sup>C]galactosamine into serum proteins and tissues of the rat was studied. After single intraperitoneal injections, about 20% of administered radioactivity was excreted in urine within 6 h. Only 1% was found in expired CO<sub>2</sub> at this time; 12% was found at 72 h. High levels of bound (non-dialyzable) radioactivity appeared first in liver and then in serum. Non-dialyzable radioactivity in serum was found associated with all electrophoretically separated fractions with the greatest concentration in the α-globulins. Hexosamines were separated by paper chromatography from serum protein hydrolysates; glucosamine had a <sup>14</sup>C content over ten times that of galactosamine in a sample taken 6 h after injection. A progressive increase in non-dialyzable radioactivity with time was found in both connective tissue and the fluid from within stainless-steel wire mesh cylinders implanted subcutaneously 2 weeks prior to injection of [1-<sup>14</sup>C]galactosamine. Mucopolysaccharides, extracted from connective tissue, exhibited electrophoretic mobility and staining characteristic of chondroitin sulfate and hyaluronic acid. Although hyaluronic acid and chondroitin sulfate moieties were labeled to the same extent, their hydrolysates contained glucosamine with 4 times more radioactivity then galactosamine, possibly indicating the presence of a substance containing glucosamine in the chondroitin sulfate area. These results indicate that much of the galactosamine is converted to glucosamine. Radioactive galactosamine appears to be of value for metabolic studies of glycoproteins and mucopolysaccharides.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90003-5","citationCount":"25","resultStr":"{\"title\":\"Incorporation of D-[1-14C]galactosamine into serum proteins and tissues of the rat\",\"authors\":\"Betty N. White ,&nbsp;M.R. Shetlar ,&nbsp;H.M. Shurley ,&nbsp;J.A. Schilling\",\"doi\":\"10.1016/0926-6534(65)90003-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Incorporation of <span>D</span>-[1-<sup>14</sup>C]galactosamine into serum proteins and tissues of the rat was studied. After single intraperitoneal injections, about 20% of administered radioactivity was excreted in urine within 6 h. Only 1% was found in expired CO<sub>2</sub> at this time; 12% was found at 72 h. High levels of bound (non-dialyzable) radioactivity appeared first in liver and then in serum. Non-dialyzable radioactivity in serum was found associated with all electrophoretically separated fractions with the greatest concentration in the α-globulins. Hexosamines were separated by paper chromatography from serum protein hydrolysates; glucosamine had a <sup>14</sup>C content over ten times that of galactosamine in a sample taken 6 h after injection. A progressive increase in non-dialyzable radioactivity with time was found in both connective tissue and the fluid from within stainless-steel wire mesh cylinders implanted subcutaneously 2 weeks prior to injection of [1-<sup>14</sup>C]galactosamine. Mucopolysaccharides, extracted from connective tissue, exhibited electrophoretic mobility and staining characteristic of chondroitin sulfate and hyaluronic acid. Although hyaluronic acid and chondroitin sulfate moieties were labeled to the same extent, their hydrolysates contained glucosamine with 4 times more radioactivity then galactosamine, possibly indicating the presence of a substance containing glucosamine in the chondroitin sulfate area. These results indicate that much of the galactosamine is converted to glucosamine. Radioactive galactosamine appears to be of value for metabolic studies of glycoproteins and mucopolysaccharides.</p></div>\",\"PeriodicalId\":100163,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1965-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6534(65)90003-5\",\"citationCount\":\"25\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926653465900035\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926653465900035","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 25

摘要

研究了D-[1-14C]半乳糖胺在大鼠血清蛋白和组织中的掺入。单次腹腔注射后,约20%的放射性物质在6小时内随尿液排出,此时在过期CO2中仅发现1%;72 h时发现12%。高水平的结合(不可透析)放射性首先出现在肝脏,然后出现在血清中。血清中不可透析的放射性与所有电泳分离组分均相关,α-球蛋白浓度最高。用纸层析法从血清蛋白水解物中分离己糖胺;在注射后6小时的样品中,葡萄糖胺的14C含量是半乳糖胺的十倍以上。在注射[1-14C]半乳糖胺前2周,结缔组织和皮下植入的不锈钢丝网筒内的液体中,发现不可透析的放射性随时间逐渐增加。从结缔组织中提取的粘多糖表现出硫酸软骨素和透明质酸的电泳迁移性和染色特性。虽然透明质酸和硫酸软骨素部分被标记的程度相同,但它们的水解产物中含有葡萄糖胺,其放射性是半乳糖胺的4倍,这可能表明硫酸软骨素区域存在含有葡萄糖胺的物质。这些结果表明,大部分半乳糖胺转化为葡萄糖胺。放射性半乳糖胺似乎对糖蛋白和粘多糖的代谢研究有价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Incorporation of D-[1-14C]galactosamine into serum proteins and tissues of the rat

Incorporation of D-[1-14C]galactosamine into serum proteins and tissues of the rat was studied. After single intraperitoneal injections, about 20% of administered radioactivity was excreted in urine within 6 h. Only 1% was found in expired CO2 at this time; 12% was found at 72 h. High levels of bound (non-dialyzable) radioactivity appeared first in liver and then in serum. Non-dialyzable radioactivity in serum was found associated with all electrophoretically separated fractions with the greatest concentration in the α-globulins. Hexosamines were separated by paper chromatography from serum protein hydrolysates; glucosamine had a 14C content over ten times that of galactosamine in a sample taken 6 h after injection. A progressive increase in non-dialyzable radioactivity with time was found in both connective tissue and the fluid from within stainless-steel wire mesh cylinders implanted subcutaneously 2 weeks prior to injection of [1-14C]galactosamine. Mucopolysaccharides, extracted from connective tissue, exhibited electrophoretic mobility and staining characteristic of chondroitin sulfate and hyaluronic acid. Although hyaluronic acid and chondroitin sulfate moieties were labeled to the same extent, their hydrolysates contained glucosamine with 4 times more radioactivity then galactosamine, possibly indicating the presence of a substance containing glucosamine in the chondroitin sulfate area. These results indicate that much of the galactosamine is converted to glucosamine. Radioactive galactosamine appears to be of value for metabolic studies of glycoproteins and mucopolysaccharides.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Subject index Erratum Author index Hexosamine and acid glycosaminoglycans in human teeth Acid mucopolysaccharides of tadpole tail fin and back skin
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1