{"title":"固态发酵生产葡萄糖淀粉酶的改进","authors":"Ashok Pandey","doi":"10.1016/0269-7483(90)90140-N","DOIUrl":null,"url":null,"abstract":"<div><p>Solid-state fermentation of wheat bran by <em>Aspergillus niger</em> for glucoamylase production was studied. Whole dough containing spores, and wheat bran, was used as inoculum and a 5% (w/w) level was found optimum for enzyme production. Improved nutrients supplementation, addition of whole corn (yellow) flour and better process controls resulted in a 5–6-fold increase in enzyme yield over preliminary results from laboratory-flask experiments. The productivity was about 520–560 IU enzyme per gram dry substrate in 96 h. When scaled-up in tray fermenters, about 80% enzyme production was achieved in comparison to flask experiments, in 36 h.</p></div>","PeriodicalId":100177,"journal":{"name":"Biological Wastes","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0269-7483(90)90140-N","citationCount":"56","resultStr":"{\"title\":\"Improvements in solid-state fermentation for glucoamylase production\",\"authors\":\"Ashok Pandey\",\"doi\":\"10.1016/0269-7483(90)90140-N\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Solid-state fermentation of wheat bran by <em>Aspergillus niger</em> for glucoamylase production was studied. Whole dough containing spores, and wheat bran, was used as inoculum and a 5% (w/w) level was found optimum for enzyme production. Improved nutrients supplementation, addition of whole corn (yellow) flour and better process controls resulted in a 5–6-fold increase in enzyme yield over preliminary results from laboratory-flask experiments. The productivity was about 520–560 IU enzyme per gram dry substrate in 96 h. When scaled-up in tray fermenters, about 80% enzyme production was achieved in comparison to flask experiments, in 36 h.</p></div>\",\"PeriodicalId\":100177,\"journal\":{\"name\":\"Biological Wastes\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0269-7483(90)90140-N\",\"citationCount\":\"56\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biological Wastes\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/026974839090140N\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Wastes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/026974839090140N","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Improvements in solid-state fermentation for glucoamylase production
Solid-state fermentation of wheat bran by Aspergillus niger for glucoamylase production was studied. Whole dough containing spores, and wheat bran, was used as inoculum and a 5% (w/w) level was found optimum for enzyme production. Improved nutrients supplementation, addition of whole corn (yellow) flour and better process controls resulted in a 5–6-fold increase in enzyme yield over preliminary results from laboratory-flask experiments. The productivity was about 520–560 IU enzyme per gram dry substrate in 96 h. When scaled-up in tray fermenters, about 80% enzyme production was achieved in comparison to flask experiments, in 36 h.