{"title":"甲基胆蒽诱导大鼠肝脏酶活性的机制研究","authors":"Harry V. Gelboin, L. Sokoloff","doi":"10.1016/0926-6550(64)90176-8","DOIUrl":null,"url":null,"abstract":"<div><p>The administration <em>in vivo</em> of 3-methylcholanthrene (MC) increases the rate of leucine incorporation in a nuclei-free rat-liver homogenate system. Each subcellular fraction, the mitochondria, microsomes and supernatant fluid contribute to the increased activity in the MC preparation. The major part of the incorporation takes place in the microsomal fraction. The increase in incorporation in the microsomes from MC-treated rats is not secondary to changes in the concentration of magnesium ion or GTP. Parallel changes in the activity of the supernatant fluid appear due to a more adequate maintenance of proper GTP levels in the supernatant from MC-treated rats.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 122-129"},"PeriodicalIF":0.0000,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90176-8","citationCount":"30","resultStr":"{\"title\":\"Studies on the mechanism of methylcholanthrene induction of enzyme activities of rat liver\",\"authors\":\"Harry V. Gelboin, L. Sokoloff\",\"doi\":\"10.1016/0926-6550(64)90176-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The administration <em>in vivo</em> of 3-methylcholanthrene (MC) increases the rate of leucine incorporation in a nuclei-free rat-liver homogenate system. Each subcellular fraction, the mitochondria, microsomes and supernatant fluid contribute to the increased activity in the MC preparation. The major part of the incorporation takes place in the microsomal fraction. The increase in incorporation in the microsomes from MC-treated rats is not secondary to changes in the concentration of magnesium ion or GTP. Parallel changes in the activity of the supernatant fluid appear due to a more adequate maintenance of proper GTP levels in the supernatant from MC-treated rats.</p></div>\",\"PeriodicalId\":100173,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"volume\":\"91 1\",\"pages\":\"Pages 122-129\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6550(64)90176-8\",\"citationCount\":\"30\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926655064901768\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064901768","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Studies on the mechanism of methylcholanthrene induction of enzyme activities of rat liver
The administration in vivo of 3-methylcholanthrene (MC) increases the rate of leucine incorporation in a nuclei-free rat-liver homogenate system. Each subcellular fraction, the mitochondria, microsomes and supernatant fluid contribute to the increased activity in the MC preparation. The major part of the incorporation takes place in the microsomal fraction. The increase in incorporation in the microsomes from MC-treated rats is not secondary to changes in the concentration of magnesium ion or GTP. Parallel changes in the activity of the supernatant fluid appear due to a more adequate maintenance of proper GTP levels in the supernatant from MC-treated rats.
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