过氧化物酶体:生物化学、分子生物学和遗传疾病-教学视频节目

Biochemical education Pub Date : 2000-05-01 DOI:10.1016/S0307-4412(00)00005-4

Norbert Latruffe, Shawn J. Hassell

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引用次数: 0

摘要

啮齿类动物肝脏中过氧化物酶体的增殖是几种外源药物的良好毒理学生化标志物，包括贝特类;邻苯二甲酸酯和己二酸酯;或者氯苯氧乙酸，一种除草剂。对过氧化物酶体的研究是将基础科学、生物医学和工业卫生等相关领域结合起来的一个很好的例子。一个25分钟的视频节目演示了纯化过氧化物酶体的表征技术(图1)下载:下载高分辨率图像(70KB)下载:下载全尺寸图像1. 电镜观察分离过氧化物酶体(取自大鼠肝脏×50 000)和细胞膜，免疫印迹，增殖标记物的测定，转录后水平的mRNA分析，DNA技术，细胞培养作为生物模型和计算机分析。它的目标是本科生和非生物化学高级学生在生物学。

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Peroxisomes: biochemistry, molecular biology and genetic diseases— a video programme for teaching students

Peroxisome proliferation in rodent liver is a good biochemical marker of toxicology for several classes of xenobiotics, including fibrates; phthalates and adipates; or chlorophenoxy-acetate, a herbicide. Research in peroxisomes provides a good example of the integration of fields related to basic sciences and biomedical and industrial health. A 25 min video programme illustrates techniques involved in the characterization of purified peroxisomes (Fig. 1

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Fig. 1. Electron microscope view of isolated peroxisomes (from rat liver ×50 000).

) and membranes, immunoblotting, measurement of proliferation markers, mRNA analysis at the post-transcriptional level, DNA techniques, cell cultures as biological models and computer analysis. It is aimed at undergraduates and non-biochemist advanced students in biology.

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Biochemical education

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