Dina Kačar , Carmen Schleissner , Librada M. Cañedo , Pilar Rodríguez , Fernando de la Calle , Carmen Cuevas , Beatriz Galán , José Luis García
{"title":"labrenzin通路扩张在体内产生pederin","authors":"Dina Kačar , Carmen Schleissner , Librada M. Cañedo , Pilar Rodríguez , Fernando de la Calle , Carmen Cuevas , Beatriz Galán , José Luis García","doi":"10.1016/j.mec.2022.e00198","DOIUrl":null,"url":null,"abstract":"<div><p>Pederin is a potent polyketide toxin that causes severe skin lesions in humans after contact with insects of genus <em>Paederus.</em> Due to its potent anticancer activities, pederin family compounds have raised the interest of pharmaceutical industry. Despite the extensive studies on the cluster of biosynthetic genes responsible for the production of pederin, it has not yet been possible to isolate and cultivate its bacterial endosymbiont producer. However, the marine bacterium <em>Labrenzia</em> sp. PHM005 was recently reported to produce labrenzin, the closest pederin analog. By cloning a synthetic <em>pedO</em> gene encoding one of the three <em>O</em>-methyltraferase of the pederin cluster into <em>Labrenzia</em> sp. PHM005 we have been able to produce pederin for the first time by fermentation in the new recombinant strain.</p></div>","PeriodicalId":18695,"journal":{"name":"Metabolic Engineering Communications","volume":"14 ","pages":"Article e00198"},"PeriodicalIF":3.7000,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214030122000074/pdfft?md5=5dd8f3cd4cbadeb5d281286c91f9e536&pid=1-s2.0-S2214030122000074-main.pdf","citationCount":"3","resultStr":"{\"title\":\"In vivo production of pederin by labrenzin pathway expansion\",\"authors\":\"Dina Kačar , Carmen Schleissner , Librada M. Cañedo , Pilar Rodríguez , Fernando de la Calle , Carmen Cuevas , Beatriz Galán , José Luis García\",\"doi\":\"10.1016/j.mec.2022.e00198\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Pederin is a potent polyketide toxin that causes severe skin lesions in humans after contact with insects of genus <em>Paederus.</em> Due to its potent anticancer activities, pederin family compounds have raised the interest of pharmaceutical industry. Despite the extensive studies on the cluster of biosynthetic genes responsible for the production of pederin, it has not yet been possible to isolate and cultivate its bacterial endosymbiont producer. However, the marine bacterium <em>Labrenzia</em> sp. PHM005 was recently reported to produce labrenzin, the closest pederin analog. By cloning a synthetic <em>pedO</em> gene encoding one of the three <em>O</em>-methyltraferase of the pederin cluster into <em>Labrenzia</em> sp. PHM005 we have been able to produce pederin for the first time by fermentation in the new recombinant strain.</p></div>\",\"PeriodicalId\":18695,\"journal\":{\"name\":\"Metabolic Engineering Communications\",\"volume\":\"14 \",\"pages\":\"Article e00198\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2022-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214030122000074/pdfft?md5=5dd8f3cd4cbadeb5d281286c91f9e536&pid=1-s2.0-S2214030122000074-main.pdf\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Metabolic Engineering Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214030122000074\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Metabolic Engineering Communications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214030122000074","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
In vivo production of pederin by labrenzin pathway expansion
Pederin is a potent polyketide toxin that causes severe skin lesions in humans after contact with insects of genus Paederus. Due to its potent anticancer activities, pederin family compounds have raised the interest of pharmaceutical industry. Despite the extensive studies on the cluster of biosynthetic genes responsible for the production of pederin, it has not yet been possible to isolate and cultivate its bacterial endosymbiont producer. However, the marine bacterium Labrenzia sp. PHM005 was recently reported to produce labrenzin, the closest pederin analog. By cloning a synthetic pedO gene encoding one of the three O-methyltraferase of the pederin cluster into Labrenzia sp. PHM005 we have been able to produce pederin for the first time by fermentation in the new recombinant strain.
期刊介绍:
Metabolic Engineering Communications, a companion title to Metabolic Engineering (MBE), is devoted to publishing original research in the areas of metabolic engineering, synthetic biology, computational biology and systems biology for problems related to metabolism and the engineering of metabolism for the production of fuels, chemicals, and pharmaceuticals. The journal will carry articles on the design, construction, and analysis of biological systems ranging from pathway components to biological complexes and genomes (including genomic, analytical and bioinformatics methods) in suitable host cells to allow them to produce novel compounds of industrial and medical interest. Demonstrations of regulatory designs and synthetic circuits that alter the performance of biochemical pathways and cellular processes will also be presented. Metabolic Engineering Communications complements MBE by publishing articles that are either shorter than those published in the full journal, or which describe key elements of larger metabolic engineering efforts.