一种双功能Ti-Ga合金:通过干扰铁代谢抑制生物膜形成和破骨细胞发生分化。

IF 11.3 1区 医学 Q1 Medicine Biomaterials Research Pub Date : 2023-03-29 DOI:10.1186/s40824-023-00362-1
Fupeng Li, Kai Huang, Jinbing Wang, Kai Yuan, Yiqi Yang, Yihao Liu, Xianhao Zhou, Keyu Kong, Tao Yang, Jian He, Chunjie Liu, Haiyong Ao, Fengxiang Liu, Qian Liu, Tingting Tang, Shengbing Yang
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引用次数: 2

摘要

背景:尽管生物医学植入物在骨科治疗中得到了广泛的应用,但仍有两个主要的临床挑战有待解决,一是细菌感染导致生物膜的形成,二是植入过程中由于破骨细胞生成过度激活导致无菌性松动。这些因素会引起许多临床问题,甚至导致种植体失败。因此,有必要赋予种植体抗生物膜和无菌防松动的特性,以促进种植体与骨组织的结合,从而成功种植。为了实现这一目标,本研究旨在以镓(Ga)为成分,开发具有抗生物膜和抗无菌松动双重功能的生物相容性钛合金。方法:制备一系列Ti-Ga合金。我们在体外和体内测试了Ga含量、Ga分布、硬度、拉伸强度、生物相容性和抗生物膜性能。我们还探讨了Ga3+离子如何抑制金黄色葡萄球菌(S. aureus)和大肠杆菌(E. coli)的生物膜形成和破骨细胞分化。结果:该合金在体外对金黄色葡萄球菌和大肠杆菌均表现出良好的抗菌膜性能,在体内对金黄色葡萄球菌均表现出良好的抗菌膜性能。蛋白质组学结果表明,Ga3+离子可以干扰金黄色葡萄球菌和大肠杆菌的细菌铁代谢,抑制细菌生物膜的形成。此外,Ti-Ga合金可以通过靶向铁代谢抑制核因子-κB配体受体激活因子(RANKL)依赖性破骨细胞分化和功能,进而抑制NF-κB信号通路的激活,从而显示其预防无菌性松动的潜力。结论:本研究提供了一种先进的Ti-Ga合金,可以作为一种有前途的骨科种植原料用于各种临床情况。这项工作还揭示了铁代谢是Ga3+离子抑制生物膜形成和破骨细胞分化的共同目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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A dual functional Ti-Ga alloy: inhibiting biofilm formation and osteoclastogenesis differentiation via disturbing iron metabolism.

Background: Although biomedical implants have been widely used in orthopedic treatments, two major clinical challenges remain to be solved, one is the bacterial infection resulting in biofilm formation, and the other is aseptic loosening during implantation due to over-activated osteoclastogenesis. These factors can cause many clinical issues and even lead to implant failure. Thus, it is necessary to endow implants with antibiofilm and aseptic loosening-prevention properties, to facilitate the integration between implants and bone tissues for successful implantation. To achieve this goal, this study aimed to develop a biocompatible titanium alloy with antibiofilm and anti-aseptic loosening dual function by utilizing gallium (Ga) as a component.

Methods: A series of Ti-Ga alloys were prepared. We examined the Ga content, Ga distribution, hardness, tensile strength, biocompatibility, and anti-biofilm performance in vitro and in vivo. We also explored how Ga3+ ions inhibited the biofilm formation of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) and osteoclast differentiation.

Results: The alloy exhibited outstanding antibiofilm properties against both S. aureus and E. coli in vitro and decent antibiofilm performance against S. aureus in vivo. The proteomics results demonstrated that Ga3+ ions could disturb the bacterial Fe metabolism of both S. aureus and E. coli, inhibiting bacterial biofilm formation. In addition, Ti-Ga alloys could inhibit receptor activator of nuclear factor-κB ligand (RANKL)-dependent osteoclast differentiation and function by targeting iron metabolism, then suppressing the activation of the NF-κB signaling pathway, thus, showing their potential to prevent aseptic loosening.

Conclusion: This study provides an advanced Ti-Ga alloy that can be used as a promising orthopedic implant raw material for various clinical scenarios. This work also revealed that iron metabolism is the common target of Ga3+ ions to inhibit biofilm formation and osteoclast differentiation.

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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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