中国南方黄木蛾的形态与分子鉴定(半翅目:蚁科)

Q2 Agricultural and Biological Sciences Grain Oil Science and Technology Pub Date : 2021-03-01 DOI:10.1016/j.gaost.2020.11.003
Zonglin Wu , Shaohua Lu , Jiying Li , Shiyuan Miao , Yujie Lu
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引用次数: 1

摘要

在对粮库天敌昆虫的调查中,我们发现一种蚁科昆虫占天敌昆虫的比例很高。从形态学和分子生物学两方面对害虫进行鉴定,以期利用害虫更准确有效地防治仓储害虫。超深度显微镜(VHX-5000)成像显示,成虫颜色为黑棕色;母天线和公天线均为四段,从基部到末端依次增加,母天线和公天线的长度分别为756.2 μm和741.1 μm。雌性和雄性昆虫的讲台均由3段组成,雄性的讲台(596.8 μm)短于雌性的讲台(734.6 μm),雌性的讲台前2段明显长于雄性。这种昆虫的腹部两侧对称,雌性的腹部比雄性的腹部宽,雄性昆虫的左腹部的第八和第九节之间有一个凹痕,它的雄性生殖器就在那里。此外,这些若虫整体呈橘黄色,卵呈椭圆形,表面有乳白色光泽和网格图案。利用DNA条形码技术对昆虫的细胞色素C氧化酶I (COI)基因序列进行鉴定,并将鉴定的COI基因序列提交Genbank数据库进行Blast比对,结果表明,COI基因序列与Genbank数据库中的X. flavipes记录具有高度的相似性(99%)。因此,我们可以确定该昆虫是X. flavipes。此外,采用邻居连接法(NJ)构建进化树,并对每个分支的置信度进行了1000次重复测试。通过系统发育树的分析,我们发现黄蝇属木蝇属。基于形态学和COI序列鉴定,我们确定采集到的天敌昆虫为黄蝇。这是第一次从形态学和分子生物学两方面对黄皮蝇的鉴定进行研究。研究结果有助于鉴定和区分花虫的种类,有利于更好地应用黄缕菊进行生物防治。
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Morphological and molecular identification of Xylocoris flavipes (Hemiptera: Anthocoridae) in southern China

During the investigation of natural enemy insects in grain depots, we've found an Anthocoridae insect accounted for a high proportion of natural enemy insects. We identify the enemy insects from morphology and molecular biology, so as to realize more accurate and effective control of storage pests by using the enemy insect. The images of the insects under the ultra-depth microscope (VHX-5000) showed that the insect adults were black brown in colour; the antennas of both the female and male had four segments, which increase in length sequentially from the base to the end, and had a length of 756.2 μm for the female and 741.1 μm for the male. The rostrums of both the female and the male insects were composed of three segments, the male's rostrum (596.8 μm) were shorter than the female's (734.6 μm), and the former two segments of the female's rostrum were significantly longer than the male's. The abdomen of the insect was bilaterally symmetrical, the female's abdomen was wider than the male, and the male insect had a dent between its eighth and ninth segments of the left abdomen, in which its male genitalia was located. In addition, the nymphs as a whole were tangerine in colour, and the eggs were elliptic with creamy white gloss and gridding patterns on the surface. The cytochrome C oxidase I (COI)  gene sequences of the insects were identified by DNA barcoding technique, and the determined COI sequences were then submitted to the Genbank database for Blast alignment, and the results showed that the COI gene sequences had a high degree of similarity (99%) with X. flavipes records in the Genbank database. Therefore, we could determine that the insect was X. flavipes. In addition, the neighbor joining (NJ) was used to construct the evolutionary tree, and 1,000 repeated tests were conducted on the confidence levels of each branch. Based on the phylogenetic tree, we've found that X. flavipes belongs to Xylocoris genus. Based on morphology and COI sequence identification, we confidentiy determined that the collected natural enemy insect was X. flavipes. This is the first time that the identification of X. flavipes is being investigated from morphology and molecular biology. The research results are helpful to identify and distinguish the species of flower bugs, and beneficial for the better application of X. flavipes in biological control.

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来源期刊
CiteScore
7.30
自引率
0.00%
发文量
69
审稿时长
12 weeks
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