Nadiah Abu, Nurul Ainaa Adilah Rus Bakarurraini, Siti Nurmi Nasir, Muhiddin Ishak, Rashidah Baharuddin, Rahman Jamal, Nurul Syakima Ab Mutalib
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引用次数: 0
摘要
背景:睾丸癌抗原(cta)是一类免疫刺激抗原，在许多类型的癌症中经常过度表达。cta作为免疫治疗靶点的使用已被广泛研究用于不同的癌症，包括黑色素瘤、血液系统恶性肿瘤和结直肠癌。研究表明cta的表观遗传调控如甲基化状态可能影响cta的表达。然而，关于cta甲基化状态的报告是相互矛盾的。cta的一般甲基化谱，特别是在结直肠癌中，仍然是难以捉摸的。目的:确定结直肠癌患者所选择的cta的甲基化谱。方法:采用Infinium Human methylation 450K芯片对54对结直肠癌样本进行DNA甲基化分析。结果:我们发现大多数cta是低甲基化的，CCNA1和TMEM108基因是少数高甲基化的cta。结论:总的来说，我们的简短报告已经成功地显示了结直肠癌中超过200种cta的总体甲基化谱，这可以用于进一步完善任何免疫治疗靶点。
Methylation Profile of Cancer Testis Antigens in Colorectal Cancer.
Background: Cancer testis antigens (CTAs) are a class of immune-stimulating antigens often overexpressed in many types of cancers. The usage of the CTAs as immunotherapy targets have been widely investigated in different cancers including melanoma, hematological malignancies, and colorectal cancer. Studies have indicated that the epigenetic regulation of the CTAs such as the methylation status may affect the expression of the CTAs. However, the report on the methylation status of the CTAs is conflicting. The general methylation profile of the CTAs, especially in colorectal cancer, is still elusive.
Objective: To determine the methylation profile of the selected CTAs in our colorectal cancer patients.
Methods: A total of 54 pairs of colorectal cancer samples were subjected to DNA methylation profiling using the Infinium Human Methylation 450K bead chip.
Results: We found that most of the CTAs were hypomethylated, and CCNA1 and TMEM108 genes were among the few CTAs that were hypermethylated.
Conclusion: Overall, our brief report has managed to show the overall methylation profile in over the 200 CTAs in colorectal cancer and this could be used for further refining any immunotherapy targets.
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The Iranian Journal of Immunology (I.J.I) is an internationally disseminated peer-reviewed publication and publishes a broad range of experimental and theoretical studies concerned with all aspects of immunology.
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