高岭土活化的再钙化柠檬酸全血在点护理粘弹性凝血试验。

IF 1.1 4区 农林科学 Q2 Veterinary Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2022-10-01
Amy M Molitoris, Armelle M deLaforcade, Alexandra Pfaff, Elizabeth A Rozanski
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引用次数: 0

摘要

粘弹性凝血监测仪(VCM)被描述为一种即时分析仪,依靠通过两块玻璃板之间的接触激活新鲜全血(FWB)。高岭土在血栓弹性成像中用作活化剂,以减少变异性和缩短凝血时间。本研究的目的是比较高岭土活化,重钙化柠檬酸样品的VCM结果与FWB的VCM结果。VCM测试使用FWB和高岭土活化、重钙化的柠檬酸样品进行。记录凝块时间(CT;秒),凝块形成时间(CFT;秒),alpha(度),振幅在10和20分钟(A10和A20;VCM单位),最大凝块硬度(MCF;VCM单位)和裂解指数(LI;%)。采用t检验或Wilcoxon-Mann-Whitney检验比较,p值< 0.05为显著性。使用Levene检验计算样本间的可变性。与FWB相比,VCM高岭土活化显著提高了CT和CFT (P < 0.0001), α角(P < 0.001)和A10和A20 (P = 0.007, P = 0.015)。MCF、LI30和LI45没有差异。在可变性方面没有发现差异。将高岭土添加到重钙化柠檬酸全血VCM样品中,可使FWB单独凝血速度更快,可考虑用于狗的临床应用。
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Kaolin activation of recalcified citrated whole blood in a point-of-care viscoelastic coagulation test.

The viscoelastic coagulation monitor (VCM) is described as a point-of-care analyzer relying on activation of fresh whole blood (FWB) via contact between 2 glass plates. Kaolin is used as an activator in thromboelastography to reduce variability and shorten clotting times. The goal of this study was to compare VCM results from kaolin-activated, recalcified citrated samples with that from FWB. The VCM testing was performed using FWB and kaolin-activated, recalcified citrated samples. The VCM results were recorded for clot time (CT; seconds), clot formation time (CFT; seconds), alpha (degree), amplitude at 10 and 20 minutes (A10 and A20; VCM units), maximum clot firmness (MCF; VCM units), and lysis index (LI; %). Values were compared using a t-test or Wilcoxon-Mann-Whitney test, with a P-value < 0.05 considered significant. Variability between samples was calculated using Levene's test. The VCM kaolin activation resulted in significantly faster CT and CFT (P < 0.0001), higher alpha angle (P < 0.001), and higher A10 and A20 (P = 0.007, P = 0.015) compared to FWB. There was no difference in MCF, LI30, or LI45. There was no difference in variability identified. The addition of kaolin to recalcified citrated whole blood VCM samples results in more rapid clotting of FWB alone and could be considered for clinical use in dogs.

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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
58
审稿时长
>24 weeks
期刊介绍: The Canadian Journal of Veterinary Research, published by the Canadian Veterinary Medical Association, is Canada''s only veterinary research publication. This quarterly peer-reviewed online-only journal has earned a wide international readership through the publishing of high quality scientific papers in the field of veterinary medicine. The Journal publishes the results of original research in veterinary and comparative medicine.
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