狮子尿通过激活SHH/GLI信号通路,恢复肠道微生物群和微生物代谢稳态,调节慢性和不可预测轻度应激大鼠海马神经再生。

IF 3 4区 医学 Q2 NEUROSCIENCES Neural Plasticity Pub Date : 2023-01-01 DOI:10.1155/2023/1455634
Pan Meng, Xi Zhang, Dandan Li, Hui Yang, Xiaoyuan Lin, Hongqing Zhao, Ping Li, Yuhong Wang, Xiaoye Wang, Jinwen Ge
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引用次数: 1

摘要

抑郁症是一种非常普遍和异质性的疾病,需要新的策略来克服抑郁症。在这项研究中,我们旨在研究狮子尿是否通过SHH/GLI信号通路调节慢性和不可预测轻度应激(CUMS)大鼠的海马神经再生,并恢复肠道微生物群和微生物代谢稳态。建立CUMS大鼠模型并给予leonurine处理。记录大鼠体重,并进行一系列试验。Western blot检测海马组织中BDNF和5-HT的表达。然后用qRT-PCR和western blot检测SHH、GLI、PTCH和SMO的表达。采用IF评价BrdU+DCX和BrdU+NeuN的共定位。采用16S rDNA高通量测序技术检测肠道菌群组成和分布。通过非靶向代谢组学分析差异代谢物。采用Pearson相关系数分析肠道菌群与微生物代谢物的相关性。CUMS造模后,大鼠体重下降,BDNF和5-HT表达下降,体重恢复,leonurine处理后BDNF和5-HT表达升高。Leonurine逆转了CUMS模型诱导的BrdU+DCX和BrdU+NeuN共定位的降低以及SHH、GLI、PTCH和SMO水平的降低。Leonurine还能恢复CUMS大鼠肠道菌群和微生物代谢物的稳态。此外,prevotellaceae_ga6a1组与3-氧胆酸、营养胆酸、胆酸呈负相关。总的来说,leonurine通过激活SHH/GLI信号通路和恢复肠道微生物群和微生物代谢稳态来调节CUMS大鼠海马神经再生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Leonurine Regulates Hippocampal Nerve Regeneration in Rats with Chronic and Unpredictable Mild Stress by Activating SHH/GLI Signaling Pathway and Restoring Gut Microbiota and Microbial Metabolic Homeostasis.

Depression is a highly prevalent and heterogeneous disorder that requires new strategies to overcome depression. In this study, we aimed to investigate whether leonurine modulated hippocampal nerve regeneration in chronic and unpredictable mild stress (CUMS) rats through the SHH/GLI signaling pathway and restoring gut microbiota and microbial metabolic homeostasis. The CUMS rat model was constructed and treated with leonurine. The body weight of rats was recorded, and a series of tests were performed. Western blot was utilized to measure the expression of BDNF and 5-HT in the hippocampus. Then the expression of SHH, GLI, PTCH, and SMO were measured by qRT-PCR and western blot. The colocalization of BrdU+DCX and BrdU+NeuN was evaluated by IF. 16S rDNA high-throughput sequencing was applied to detect the composition and distribution of gut microbiota. The differential metabolites were analyzed by untargeted metabolomics. The correlation between gut microbiota and microbial metabolites was analyzed by Pearson correlation coefficient. After CUMS modeling, the body weight of rats was decreased, and the expression of BDNF and 5-HT were decreased, while the body weight was recovered, and the expression of BDNF and 5-HT were increased after leonurine treatment. Leonurine reversed the reduction in the colocalization of BrdU+DCX and BrdU+NeuN and the reduction in the levels of SHH, GLI, PTCH, and SMO induced by CUMS modeling. Leonurine also restored gut microbiota and microbial metabolites homeostasis in CUMS rats. Furthermore, Prevotellaceae_Ga6A1_group was negatively correlated with 3-Oxocholic acid, nutriacholic acid, and cholic acid. Collectively, leonurine regulated hippocampal nerve regeneration in CUMS rats by activating the SHH/GLI signaling pathway and restoring gut microbiota and microbial metabolic homeostasis.

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来源期刊
Neural Plasticity
Neural Plasticity NEUROSCIENCES-
CiteScore
6.80
自引率
0.00%
发文量
77
审稿时长
16 weeks
期刊介绍: Neural Plasticity is an international, interdisciplinary journal dedicated to the publication of articles related to all aspects of neural plasticity, with special emphasis on its functional significance as reflected in behavior and in psychopathology. Neural Plasticity publishes research and review articles from the entire range of relevant disciplines, including basic neuroscience, behavioral neuroscience, cognitive neuroscience, biological psychology, and biological psychiatry.
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