lncRNA FOXD2-AS1表达作为结直肠癌诊断生物标志物的评价

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2022-10-01 DOI:10.52547/rbmb.11.3.471
Hooman Shalmashi, Sahar Safaei, Dariush Shanehbandi, Milad Asadi, Soghra Bornehdeli, Abdolreza Mehdi Navaz
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引用次数: 0

摘要

背景:结直肠癌(CRC)仍被认为是世界范围内常见的癌症之一。研究早期发现结直肠癌的潜在生物标志物对于使用治疗策略有效管理患者至关重要。因此,本研究旨在探讨lncRNA FOXD2-AS1在结直肠肿瘤发生过程中的表达变化。方法:制备50例结直肠癌肿瘤组织和50例癌旁正常组织标本。从样品中提取总RNA,然后逆转录成互补DNA。接下来,利用实时荧光定量PCR技术评估lncRNA FOXD2-AS1在结直肠癌样本中与正常样本的表达水平。采用受试者工作特征曲线分析评价FOXD2-AS1对CRC的诊断价值。结果:得到的结果显示FOXD2-AS1基因的表达水平显著(p)。结论:综上所述,本研究提示组织特异性上调lncRNA FOXD2-AS1可能是CRC合适的诊断生物标志物。然而,需要更多的研究来验证这些结果,并进一步阐明FOXD2-AS1在结直肠肿瘤发生中的功能。
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Evaluation of lncRNA FOXD2-AS1 Expression as a Diagnostic Biomarker in Colorectal Cancer.

Background: Colorectal cancer (CRC) is still considered one of the prevalent cancers worldwide. Investigation of potential biomarkers for early detection of CRC is essential for the effective management of patients using therapeutic strategies. Considering that, this study was aimed to examine the changes in lncRNA FOXD2-AS1 expression through colorectal tumorigenesis.

Methods: Fifty CRC tumor tissues and fifty adjacent normal tissue samples were prepared and involved in the current study. Total RNA was extracted from the samples and then reverse transcribed to complementary DNA. Next, the expression levels of lncRNA FOXD2-AS1 were evaluated using real-time PCR in CRC samples compared to normal ones. Also, receiver operating characteristic curve analysis was used to evaluate the diagnostic value of FOXD2-AS1 for CRC.

Results: The obtained results showed that the expression level of FOXD2-AS1 gene was significantly (p<0.0001) up-regulated in tumor tissues compared to normal marginal tissues. Also, a significant correlation was observed between higher the expression of FOXD2-AS1and the differentiation of tumor cells. Furthermore, ROC curve analysis estimated an AUC value of 0.59 for FOXD2-AS1, suggesting its potential as a diagnostic target.

Conclusion: Taken together, the current study implied that tissue-specific upregulation of lncRNA FOXD2-AS1 might be appropriate diagnostic biomarkers for CRC. Nonetheless, more studies are needed to validate these results and further illustrate FOXD2-AS1 function through colorectal tumorigenesis.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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