临床患者尿液与生殖器标本检测人乳头瘤病毒(HPV)的比较。

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY Obstetrics and Gynecology International Pub Date : 2023-01-01 DOI:10.1155/2023/7483783
Hui Yang, Zhao-Yun Luo, Fen Lin, Lie-Jun Li, Min Lu, Long-Xu Xie, Li-Ye Yang
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引用次数: 0

摘要

背景:人乳头瘤病毒(HPV)是宫颈癌的主要病因。本研究的目的是研究配对生殖器和尿液样本的HPV DNA检测和基因分型,并评估尿液样本是否可以用于监测HPV感染。方法:研究对象于2011年9月1日至2012年6月30日在中国广东省一家地方医院招募。如果他们在3-5天前接受了HPV基因分型检测以进行生殖-泌尿系统疾病的临床诊断或进行了健康检查,他们就被邀请参加。从配对的生殖器和尿液样本中提取DNA;采用GenoArray法进行基因分型。结果:共纳入患者250例,其中女性203例,男性47例。我们的结果显示,配对样本中女性HPV状态的总体一致性为77.1% (155/201,95% CI: 0.713-0.829), kappa值为0.523 (95% CI: 0.469-0.632),而配对样本中男性HPV状态的一致性极低。在个体基因分型方面,女性HPV16型特异性鉴定一致性最高(96.02%,0.933-0.987),其余12种高危型(HR-HPV)次之,低危型HPV检测一致性较差(κ p = 0.002)。此外,低风险HPV检测的一致性明显低于HR-HPV检测(48.1%对62.3%,p = 0.044)。结论:尽管敏感性降低,但尿液中HPV检测的趋势与生殖器采样所见的趋势密切相关。尿液似乎是非常有限的医疗保健妇女HPV DNA检测的合适替代样本,而尿液用于男性HPV DNA检测的效用则不太确定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Comparison of Urine and Genital Samples for Detecting Human Papillomavirus (HPV) in Clinical Patients.

Background: Human papillomavirus (HPV) is the main cause of cervical cancer. The aim of the present study was to investigate HPV DNA detection and genotyping on paired genital and urine samples and to evaluate if urine samples could be used to monitor HPV infection.

Methods: Study subjects were recruited from one local hospital in Guangdong of China from September 1, 2011, to June 30, 2012. They were invited to participate if they have taken an HPV genotyping assay for clinical diagnosis of the genital-urinary disease or for a health check-up 3-5 days ago. DNA was extracted from paired genital and urine samples; genotyping was performed with the GenoArray assay.

Results: A total of 250 patients were recruited, which included 203 females and 47 males. Our results showed that the overall agreement on HPV status between the paired samples was 77.1% (155/201, 95% CI: 0.713-0.829) for females, with a kappa value of 0.523 (95% CI: 0.469-0.632), while the agreement was extremely low in the paired male samples. As to individual genotyping, the greatest agreement was found for HPV16 type-specific identification in females (96.02%, 0.933-0.987), followed by the other 12 high oncogenic risk (HR-HPV) types, while the agreement for low-risk HPV detection is poor (κ < 0.6). Agreement between paired samples showed that HPV detection had a significantly greater concordance in the samples obtained in females than males (p = 0.002). Moreover, the agreement for low-risk HPV detection was significantly lower as compared to HR-HPV detection (48.1% vs. 62.3%, p = 0.044).

Conclusion: Despite reduced sensitivity, HPV detection in urine closely represents the same trend that is seen with genital sampling. Urine appears to be an appropriate surrogate sample for HPV DNA detection in women with very limited access to healthcare, while the utility of urine for HPV DNA detection in males is less certain.

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来源期刊
Obstetrics and Gynecology International
Obstetrics and Gynecology International OBSTETRICS & GYNECOLOGY-
CiteScore
3.60
自引率
0.00%
发文量
26
审稿时长
19 weeks
期刊介绍: Obstetrics and Gynecology International is a peer-reviewed, Open Access journal that aims to provide a forum for scientists and clinical professionals working in obstetrics and gynecology. The journal publishes original research articles, review articles, and clinical studies related to obstetrics, maternal-fetal medicine, general gynecology, gynecologic oncology, uro-gynecology, reproductive medicine and infertility, reproductive endocrinology, and sexual medicine.
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