黑醋栗多糖可减轻高脂/高胆固醇饮食对大鼠肠道的影响。

IF 2.3 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food Technology and Biotechnology Pub Date : 2022-12-01 DOI:10.17113/ftb.60.04.22.7561
Xin Wei, Yuan Gao, Feier Cheng, Shaojun Yun, Mingchang Chang, Jinling Cao, Yanfen Cheng, Cuiping Feng
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引用次数: 1

摘要

研究背景:黑醋栗多糖具有调节血清和肝脏血脂和胆固醇的作用。然而,多糖对胆固醇代谢的调节机制,特别是与肠道菌群调节的因果关系尚不清楚。本研究将为进一步研究苦参多糖的降胆固醇机理和功能食品的开发提供理论依据。实验方法:本研究探讨黑醋栗多糖对高脂高胆固醇饮食大鼠肠道胆固醇代谢的调节机制。简单地说,酶比色微孔板法测定粪便胆汁酸的浓度。采用气相色谱-质谱联用法测定粪便中胆固醇和酒精的含量。采用苏木精和伊红染色法观察小肠组织结构的变化。采用实时荧光定量聚合酶链反应检测相关基因在空肠和回肠中的表达。Western blot检测空肠相关蛋白的表达。采用高通量测序技术检测盲肠内容物肠道菌群变化。采用气相色谱-质谱法测定盲肠内容物中短链脂肪酸的浓度。结果与结论:结果表明,黑醋栗多糖能改善高脂高胆固醇饲粮大鼠肠道形态结构和生理指标。此外,它可以通过降低HMGCR、NPC1L1、ACAT2、MTP、ASBT和IBABP mRNA或蛋白的表达,提高ABCG8 mRNA的表达,改善高脂高胆固醇饮食引起的肠道胆固醇代谢紊乱。此外,还能提高拟杆菌、丁酸球菌、拟副杆菌、副utteerella和Alloprevotella的相对丰度和短链脂肪酸浓度,全面调节肠道胆固醇代谢。代谢组学分析发现,黑檀多糖对脂质、碳水化合物等相关代谢产物有影响。一些与胆固醇代谢相关的生物标志物与特定肠道微生物群的丰度显著相关。新颖性和科学贡献:这些发现表明,黑醋栗多糖可以减轻肠道胆固醇代谢紊乱,与调节肠道微生物群和改善宿主代谢有关。这些研究结果为木犀草作为一种新的食物资源的开发提供了理论支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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The Effects of a High-Fat/Cholesterol Diet on the Intestine of Rats Were Attenuated by Sparassis latifolia Polysaccharides.

Research background: Sparassis latifolia polysaccharides can regulate lipids and cholesterol in serum and liver. However, little is known about the regulation mechanism of the polysaccharides on cholesterol metabolism and especially the causal relationship with gut microbiota regulation. This study will provide a theoretical basis for the cholesterol-lowering mechanism of S. latifolia polysaccharides and further development of functional foods.

Experimental approach: In this study, we investigated how the regulation mechanism of Sparassis latifolia polysaccharides affects intestinal cholesterol metabolism in high-fat and high-cholesterol diet-fed rats. Briefly, enzymatic colorimetric microplate assay was used to determine the concentration of faecal bile acid. Gas chromatography-mass spectrometry was used to detect the content of cholesterol and alcohol in faeces. Haematoxylin and eosin staining method was applied to observe the changes in the structure of the small intestine tissue. The related gene expressions in jejunum and ileum were detected by real-time fluorescent quantitative polymerase chain reaction. The related protein expressions in jejunum were studied by using Western blot. High-throughput sequencing was used to detect the intestinal flora changes of the caecal contents. Gas chromatography-mass spectrometry was applied to detect the concentration of short-chain fatty acids in the caecal content.

Results and conclusions: The results showed that Sparassis latifolia polysaccharides could improve the intestinal morphological structure and physiological indices in rats fed high-fat and high-cholesterol diet. Moreover, it could improve intestinal cholesterol metabolism disorder induced by high-fat and high-cholesterol diets via the reduction of the expression of HMGCR, NPC1L1, ACAT2, MTP, ASBT and IBABP mRNA or protein, increasing ABCG8 mRNA expression. In addition, it could also increase the relative abundance of Bacteroides, Butyricicoccus, Parabacteroides, Parasutteerella and Alloprevotella and the short-chain fatty acid concentration, to comprehensively regulate the intestinal cholesterol metabolism. The metabolomics analysis found that Sparassis latifolia polysaccharides could affect lipid, carbohydrate and other related metabolites. Some biomarkers associated with cholesterol metabolism correlated significantly with the abundance of specific intestinal microbiota.

Novelty and scientific contribution: These findings indicate that Sparassis latifolia polysaccharides could attenuate intestinal cholesterol metabolism disorder, correlating with modulating gut microbiota and improving host metabolism. They provide theoretical support for the development of Sparassis latifolia as a new food resource.

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来源期刊
Food Technology and Biotechnology
Food Technology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
3.70
自引率
0.00%
发文量
33
审稿时长
12 months
期刊介绍: Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment. The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.
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