IF 1.8 3区 医学Q4 BIOCHEMISTRY & MOLECULAR BIOLOGYMolecular VisionPub Date : 2022-01-01
Yuan Chen, Yixiang Zhou, Xue Zhu, Ge Yan, Donghui Pan, Lizhen Wang, Min Yang, Ke Wang
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In this study, [<sup>18</sup>F]-DPA-714 micro-PET imaging was used to evaluate retinal inflammation in mice exposed to blue light, a well-established model of age-related macular degeneration (AMD) for molecular mechanism research and drug screening.</p><p><strong>Methods: </strong>C57BL/6J melanized mice were subjected to 10,000, 15,000, and 20,000 lux blue light for 5 days (8 h/day) to develop the retinal injury model, and the structure and function of the retina were assessed using hematoxylin-eosin (HE) staining, electroretinography (ERG), and terminal-deoxynucleotidyl transferase (TdT)-mediated nick-end labeling (TUNEL) immunostaining. Then, [<sup>18</sup>F]-DPA-714 was injected approximately 100 μCi through each tail vein, and static imaging was performed 1 h after injection. Finally, the mice eyeballs were collected for biodistribution and immune analysis.</p><p><strong>Results: </strong>The blue light exposure significantly destroyed the structure and function of the retina, and the uptake of [<sup>18</sup>F]-DPA-714 in the retinas of the mice exposed to blue light were the most significantly upregulated, which was consistent with the biodistribution data. In addition, the immunohistochemical, western blot, and immunofluorescence data showed an increase in microglial TSPO expression.</p><p><strong>Conclusions: </strong>[<sup>18</sup>F]-DPA-714 micro-PET imaging might be a good method for evaluating early inflammatory status during retinal pathology.</p>","PeriodicalId":18866,"journal":{"name":"Molecular Vision","volume":"28 ","pages":"507-515"},"PeriodicalIF":1.8000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/43/d4/mv-v28-507.PMC10115360.pdf","citationCount":"0","resultStr":"{\"title\":\"PET imaging of retinal inflammation in mice exposed to blue light using [<sup>18</sup>F]-DPA-714.\",\"authors\":\"Yuan Chen, Yixiang Zhou, Xue Zhu, Ge Yan, Donghui Pan, Lizhen Wang, Min Yang, Ke Wang\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Positron emission tomography (PET) is widely used in high-precision imaging, which may provide a simple and noninvasive method for the detection of pathology and therapeutic effects. 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引用次数: 0
摘要
目的:正电子发射断层扫描(PET)广泛应用于高精度成像，为病理和治疗效果的检测提供了一种简单、无创的方法。[18F]-DPA-714是第二代转运蛋白(TSPO)正电子发射断层扫描放射性示踪剂，在神经炎症模型中显示出很大的前景。本研究采用[18F]-DPA-714微pet成像技术对蓝光照射小鼠视网膜炎症进行评估，以研究老年性黄斑变性(AMD)的分子机制和药物筛选。方法:将C57BL/6J黑色素化小鼠置于10,000、15,000、20,000勒克斯蓝光下5 d (8 h/d)建立视网膜损伤模型，采用苏木精-伊红(HE)染色、视网膜电图(ERG)和末端脱氧核苷酸转移酶(TdT)介导的镍端标记(TUNEL)免疫染色评价视网膜的结构和功能。然后通过尾静脉注射[18F]-DPA-714约100 μCi，注射1 h后进行静态成像。最后采集小鼠眼球进行生物分布和免疫分析。结果:蓝光暴露显著破坏了视网膜的结构和功能，蓝光暴露小鼠视网膜中[18F]-DPA-714的摄取上调最为显著，与生物分布数据一致。此外，免疫组织化学、免疫印迹和免疫荧光数据显示小胶质细胞TSPO表达增加。结论:[18F]-DPA-714微pet成像可能是评估视网膜病理早期炎症状态的良好方法。
PET imaging of retinal inflammation in mice exposed to blue light using [18F]-DPA-714.
Purpose: Positron emission tomography (PET) is widely used in high-precision imaging, which may provide a simple and noninvasive method for the detection of pathology and therapeutic effects. [18F]-DPA-714 is a second-generation translocator protein (TSPO) positron emission tomography radiotracer that shows great promise in a model of neuroinflammation. In this study, [18F]-DPA-714 micro-PET imaging was used to evaluate retinal inflammation in mice exposed to blue light, a well-established model of age-related macular degeneration (AMD) for molecular mechanism research and drug screening.
Methods: C57BL/6J melanized mice were subjected to 10,000, 15,000, and 20,000 lux blue light for 5 days (8 h/day) to develop the retinal injury model, and the structure and function of the retina were assessed using hematoxylin-eosin (HE) staining, electroretinography (ERG), and terminal-deoxynucleotidyl transferase (TdT)-mediated nick-end labeling (TUNEL) immunostaining. Then, [18F]-DPA-714 was injected approximately 100 μCi through each tail vein, and static imaging was performed 1 h after injection. Finally, the mice eyeballs were collected for biodistribution and immune analysis.
Results: The blue light exposure significantly destroyed the structure and function of the retina, and the uptake of [18F]-DPA-714 in the retinas of the mice exposed to blue light were the most significantly upregulated, which was consistent with the biodistribution data. In addition, the immunohistochemical, western blot, and immunofluorescence data showed an increase in microglial TSPO expression.
Conclusions: [18F]-DPA-714 micro-PET imaging might be a good method for evaluating early inflammatory status during retinal pathology.
期刊介绍：
Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical).
Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints.
For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.
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