非分段负链RNA病毒聚合酶的结构和机制。

IF 8.1 1区 医学 Q1 VIROLOGY Annual Review of Virology Pub Date : 2023-09-29 Epub Date: 2023-05-03 DOI:10.1146/annurev-virology-111821-102603
Mohamed Ouizougun-Oubari, Rachel Fearns
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引用次数: 3

摘要

非片段负链RNA病毒(nsNSV),也称为单阴性病毒目,其基因组由单链负义RNA组成。nsNSV复制周期中不可或缺的是病毒聚合酶,它负责转录病毒基因组,产生一系列带帽和多腺苷酸化的信使RNA,并将其复制以产生新的基因组。为了进行这些过程所需的不同步骤,nsNSV聚合酶经历一系列配位构象转变。尽管关于nsNSV聚合酶动力学、结构和功能的交叉还有很多需要了解,但最近发表的聚合酶结构,结合生物化学和分子生物学研究的历史,为nsNSV多聚酶如何作为动态机器发挥作用提供了新的见解。在这篇综述中,我们考虑了nsNSV转录和复制所涉及的每一个步骤,并提出了这些步骤与已解决的聚合酶结构的关系。
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Structures and Mechanisms of Nonsegmented, Negative-Strand RNA Virus Polymerases.

The nonsegmented, negative-strand RNA viruses (nsNSVs), also known as the order Mononegavirales, have a genome consisting of a single strand of negative-sense RNA. Integral to the nsNSV replication cycle is the viral polymerase, which is responsible for transcribing the viral genome, to produce an array of capped and polyadenylated messenger RNAs, and replicating it to produce new genomes. To perform the different steps that are necessary for these processes, the nsNSV polymerases undergo a series of coordinated conformational transitions. While much is still to be learned regarding the intersection of nsNSV polymerase dynamics, structure, and function, recently published polymerase structures, combined with a history of biochemical and molecular biology studies, have provided new insights into how nsNSV polymerases function as dynamic machines. In this review, we consider each of the steps involved in nsNSV transcription and replication and suggest how these relate to solved polymerase structures.

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来源期刊
CiteScore
19.40
自引率
0.90%
发文量
28
期刊介绍: The Annual Review of Virology serves as a conduit for disseminating thrilling advancements in our comprehension of viruses spanning animals, plants, bacteria, archaea, fungi, and protozoa. Its reviews illuminate novel concepts and trajectories in basic virology, elucidating viral disease mechanisms, exploring virus-host interactions, and scrutinizing cellular and immune responses to virus infection. These reviews underscore the exceptional capacity of viruses as potent probes for investigating cellular function.
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