{"title":"[十八二烯酸对胶质瘤细胞增殖和凋亡的影响及其机制]。","authors":"Ming-Ren Xie, Tian-Xiao He, Xia Yuan, Jing Zhang, Lei Yu, Fa-Rong Yu","doi":"10.12047/j.cjap.6271.2022.081","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To study the effects of octadecadienoic acid (ODA) on the proliferation and apoptosis of glioma cells and its mechanisms.</p><p><strong>Methods: </strong>Cultured human glioma cells (cell density 2×10<sup>6</sup> cells/L) were divided into solvent control group (DMSO, 30 μl/L), 5-FU group (10 mg/L) and octadecadienic acid groups (0.3, 0.6 and 1.2 mg/L groups). The toxicity of ODA on glioma cells was detected by trypan blue and thiazolium blue (MTT). The expression levels of P53, PI3K, P21, PKB/Akt and Caspase-9 in glioma cells were determined by enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>① Cell count under optical microscope showed that the inhibition rate of cell proliferation in ODA low, medium and high dose groups and 5-FU group was significantly higher than that in the solvent control group (<i>P</i><0.01), but there was no statistical significance compared with the 5-FU group (<i>P</i>>0.05). ② MTT assay showed that the inhibition rate of cell proliferation was increased significantly in ODA low, medium and high dose groups and 5-FU groups (<i>P</i><0.01), compared with the solvent control group. Compared with 5-FU group, the inhibition rate of cell proliferation was increased significantly only in ODA high dose group (<i>P</i><0.01). ③ The number of G<sub>0</sub>/G<sub>1</sub> phase cells in ODA low, medium and high dose groups and 5-FU group were increased significantly (<i>P</i><0.05, <i>P</i><0.01), the number of G<sub>2</sub>/M phase cells were decreased significantly (<i>P</i><0.01), and the apoptosis rate was increased significantly (<i>P</i><0.01),compared with the solvent control group. Compared with the 5-FU group, the number of cells in G<sub>2</sub>/M phase was decreased significantly (<i>P</i><0.01) and the apoptosis rate was increased significantly (<i>P</i><0.01) in ODA high dose group. ④ ELISA test results showed that the protein expression levels of P53, PI3K and PKB/Akt in ODA low , medium and high dose groups and 5-FU group were significantly lower than those in solvent control group (all <i>P</i><0.01), but the protein expression levels in ODA high dose group were significantly lower than those in 5-FU group (<i>P</i><0.01). The protein expression levels of P21 and caspase-9 in ODA low , medium and high dose groups and 5-FU group were significantly higher than those in solvent control group (<i>P</i><0.05, <i>P</i><0.01), but the protein expression levels in ODA high dose group were significantly higher than those in 5-Fu group (<i>P</i><0.01).</p><p><strong>Conclusion: </strong>ODA can significantly inhibit the proliferation and promote apoptosis of glioma cells. The mechanisms are related to up-regulating the levels of P21 and caspase-9 to promote apoptosis, down-regulating the levels of P53, PI3K and PKB/Akt to inhibit the cell division cycle, and reducing the activity of PI3K-Akt signal transduction pathway.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Effects of octadecadienoic acid on proliferation and apoptosis of glioma cells and its mechanisms].\",\"authors\":\"Ming-Ren Xie, Tian-Xiao He, Xia Yuan, Jing Zhang, Lei Yu, Fa-Rong Yu\",\"doi\":\"10.12047/j.cjap.6271.2022.081\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To study the effects of octadecadienoic acid (ODA) on the proliferation and apoptosis of glioma cells and its mechanisms.</p><p><strong>Methods: </strong>Cultured human glioma cells (cell density 2×10<sup>6</sup> cells/L) were divided into solvent control group (DMSO, 30 μl/L), 5-FU group (10 mg/L) and octadecadienic acid groups (0.3, 0.6 and 1.2 mg/L groups). The toxicity of ODA on glioma cells was detected by trypan blue and thiazolium blue (MTT). The expression levels of P53, PI3K, P21, PKB/Akt and Caspase-9 in glioma cells were determined by enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>① Cell count under optical microscope showed that the inhibition rate of cell proliferation in ODA low, medium and high dose groups and 5-FU group was significantly higher than that in the solvent control group (<i>P</i><0.01), but there was no statistical significance compared with the 5-FU group (<i>P</i>>0.05). ② MTT assay showed that the inhibition rate of cell proliferation was increased significantly in ODA low, medium and high dose groups and 5-FU groups (<i>P</i><0.01), compared with the solvent control group. Compared with 5-FU group, the inhibition rate of cell proliferation was increased significantly only in ODA high dose group (<i>P</i><0.01). ③ The number of G<sub>0</sub>/G<sub>1</sub> phase cells in ODA low, medium and high dose groups and 5-FU group were increased significantly (<i>P</i><0.05, <i>P</i><0.01), the number of G<sub>2</sub>/M phase cells were decreased significantly (<i>P</i><0.01), and the apoptosis rate was increased significantly (<i>P</i><0.01),compared with the solvent control group. Compared with the 5-FU group, the number of cells in G<sub>2</sub>/M phase was decreased significantly (<i>P</i><0.01) and the apoptosis rate was increased significantly (<i>P</i><0.01) in ODA high dose group. ④ ELISA test results showed that the protein expression levels of P53, PI3K and PKB/Akt in ODA low , medium and high dose groups and 5-FU group were significantly lower than those in solvent control group (all <i>P</i><0.01), but the protein expression levels in ODA high dose group were significantly lower than those in 5-FU group (<i>P</i><0.01). The protein expression levels of P21 and caspase-9 in ODA low , medium and high dose groups and 5-FU group were significantly higher than those in solvent control group (<i>P</i><0.05, <i>P</i><0.01), but the protein expression levels in ODA high dose group were significantly higher than those in 5-Fu group (<i>P</i><0.01).</p><p><strong>Conclusion: </strong>ODA can significantly inhibit the proliferation and promote apoptosis of glioma cells. The mechanisms are related to up-regulating the levels of P21 and caspase-9 to promote apoptosis, down-regulating the levels of P53, PI3K and PKB/Akt to inhibit the cell division cycle, and reducing the activity of PI3K-Akt signal transduction pathway.</p>\",\"PeriodicalId\":23985,\"journal\":{\"name\":\"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12047/j.cjap.6271.2022.081\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12047/j.cjap.6271.2022.081","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
[Effects of octadecadienoic acid on proliferation and apoptosis of glioma cells and its mechanisms].
Objective: To study the effects of octadecadienoic acid (ODA) on the proliferation and apoptosis of glioma cells and its mechanisms.
Methods: Cultured human glioma cells (cell density 2×106 cells/L) were divided into solvent control group (DMSO, 30 μl/L), 5-FU group (10 mg/L) and octadecadienic acid groups (0.3, 0.6 and 1.2 mg/L groups). The toxicity of ODA on glioma cells was detected by trypan blue and thiazolium blue (MTT). The expression levels of P53, PI3K, P21, PKB/Akt and Caspase-9 in glioma cells were determined by enzyme-linked immunosorbent assay (ELISA).
Results: ① Cell count under optical microscope showed that the inhibition rate of cell proliferation in ODA low, medium and high dose groups and 5-FU group was significantly higher than that in the solvent control group (P<0.01), but there was no statistical significance compared with the 5-FU group (P>0.05). ② MTT assay showed that the inhibition rate of cell proliferation was increased significantly in ODA low, medium and high dose groups and 5-FU groups (P<0.01), compared with the solvent control group. Compared with 5-FU group, the inhibition rate of cell proliferation was increased significantly only in ODA high dose group (P<0.01). ③ The number of G0/G1 phase cells in ODA low, medium and high dose groups and 5-FU group were increased significantly (P<0.05, P<0.01), the number of G2/M phase cells were decreased significantly (P<0.01), and the apoptosis rate was increased significantly (P<0.01),compared with the solvent control group. Compared with the 5-FU group, the number of cells in G2/M phase was decreased significantly (P<0.01) and the apoptosis rate was increased significantly (P<0.01) in ODA high dose group. ④ ELISA test results showed that the protein expression levels of P53, PI3K and PKB/Akt in ODA low , medium and high dose groups and 5-FU group were significantly lower than those in solvent control group (all P<0.01), but the protein expression levels in ODA high dose group were significantly lower than those in 5-FU group (P<0.01). The protein expression levels of P21 and caspase-9 in ODA low , medium and high dose groups and 5-FU group were significantly higher than those in solvent control group (P<0.05, P<0.01), but the protein expression levels in ODA high dose group were significantly higher than those in 5-Fu group (P<0.01).
Conclusion: ODA can significantly inhibit the proliferation and promote apoptosis of glioma cells. The mechanisms are related to up-regulating the levels of P21 and caspase-9 to promote apoptosis, down-regulating the levels of P53, PI3K and PKB/Akt to inhibit the cell division cycle, and reducing the activity of PI3K-Akt signal transduction pathway.