利用发育调控基因设计水稻穗部结构。

IF 2 4区 农林科学 Q2 AGRONOMY Breeding Science Pub Date : 2023-03-01 DOI:10.1270/jsbbs.22075
Ayumi Agata, Motoyuki Ashikari, Yutaka Sato, Hidemi Kitano, Tokunori Hobo
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引用次数: 1

摘要

水稻穗部结构在分枝数、分枝长度和籽粒排列上表现出显著的多样性;然而,对于这种模式的多样性是如何产生的,人们仍然知之甚少。虽然已经鉴定出几个与穗支数和穗长有关的基因,但穗支数和穗长是如何协调调控的尚不清楚。本研究表明,穗长和穗支数分别由pr15 /OsGA20ox4、Pbl6/APO1和Gn1a/OsCKX2基因独立调控。我们在Koshihikari遗传背景下获得了含有pr15精英等位基因的近等基因系(NILs),调控穗轴长度;Pbl6,调节一次支路长度;和Gn1a,调节不同组合的穗枝分枝。携带pr15、Pbl6和Gn1a的锥形系的穗长和分枝增加,但分枝长度和分枝数之间没有权衡关系。我们通过改变这三个位点的等位基因组合,成功地产生了不同的籽粒排列模式。除穗数外,改良穗型对产量相关性状无负相关影响。利用x射线计算机断层扫描(CT)对籽粒进行三维(3D)分析,发现籽粒结构的差异影响籽粒灌浆。重要的是,我们确定Prl5在不影响粒数的情况下改善了籽粒灌浆。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Designing rice panicle architecture via developmental regulatory genes.

Rice panicle architecture displays remarkable diversity in branch number, branch length, and grain arrangement; however, much remains unknown about how such diversity in patterns is generated. Although several genes related to panicle branch number and panicle length have been identified, how panicle branch number and panicle length are coordinately regulated is unclear. Here, we show that panicle length and panicle branch number are independently regulated by the genes Prl5/OsGA20ox4, Pbl6/APO1, and Gn1a/OsCKX2. We produced near-isogenic lines (NILs) in the Koshihikari genetic background harboring the elite alleles for Prl5, regulating panicle rachis length; Pbl6, regulating primary branch length; and Gn1a, regulating panicle branching in various combinations. A pyramiding line carrying Prl5, Pbl6, and Gn1a showed increased panicle length and branching without any trade-off relationship between branch length or number. We successfully produced various arrangement patterns of grains by changing the combination of alleles at these three loci. Improvement of panicle architecture raised yield without associated negative effects on yield-related traits except for panicle number. Three-dimensional (3D) analyses by X-ray computed tomography (CT) of panicles revealed that differences in panicle architecture affect grain filling. Importantly, we determined that Prl5 improves grain filling without affecting grain number.

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来源期刊
Breeding Science
Breeding Science 农林科学-农艺学
CiteScore
4.90
自引率
4.20%
发文量
37
审稿时长
1.5 months
期刊介绍: Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.
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