胎盘内注射AAV9-CMV-iCre可导致双报告小鼠胚胎中多个器官的广泛转导。

IF 2.2 4区农林科学 Q1 VETERINARY SCIENCES Experimental Animals Pub Date : 2023-11-09 Epub Date: 2023-05-12 DOI:10.1538/expanim.23-0044

Natalia Gogoleva, Zeynab Javanfekr Shahri, Atsushi Noda, Ching-Wei Liao, Arata Wakimoto, Yuri Inoue, Hyojung Jeon, Satoru Takahashi, Michito Hamada

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引用次数: 0

摘要

腺相关病毒血清型9（AAV9）由于其能够跨越血脑屏障并有效地将遗传物质转导到各种细胞类型中，已成为一种流行的基因转移工具。该研究利用了GRR（绿色到红色报告基因）小鼠胚胎，其中iCre的表达导致绿色荧光蛋白（GFP）表达的消失，并通过胎盘内注射检测到盘体虫属的红色荧光蛋白（DsRed）表达。我们的研究结果表明，AAV9-CMV-iCre可以转导胚胎E9.5-E11.5发育阶段的多个器官，包括肝脏、心脏、大脑、胸腺和肠道。这些发现表明，胎盘内注射AAV9 CMV iCre是广泛转导GRR小鼠胚胎的可行方法。

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Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos.

Adeno-associated virus serotype 9 (AAV9) has become a popular tool for gene transfer because of its ability to cross the blood-brain barrier and efficiently transduce genetic material into a variety of cell types. The study utilized GRR (Green-to-Red Reporter) mouse embryos, in which the expression of iCre results in the disappearance of Green Fluorescent Protein (GFP) expression and the detection of Discosoma sp. Red Fluorescent Protein (DsRed) expression by intraplacental injection. Our results demonstrate that AAV9-CMV-iCre can transduce multiple organs in embryos at developmental stages E9.5-E11.5, including the liver, heart, brain, thymus, and intestine. These findings suggest that intraplacental injection of AAV9-CMV-iCre is a viable method for the widespread transduction of GRR mouse embryos.

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来源期刊

Experimental Animals 生物-动物学

CiteScore

2.80

自引率

4.20%

发文量

审稿时长

3 months

期刊介绍： The aim of this international journal is to accelerate progress in laboratory animal experimentation and disseminate relevant information in related areas through publication of peer reviewed Original papers and Review articles. The journal covers basic to applied biomedical research centering around use of experimental animals and also covers topics related to experimental animals such as technology, management, and animal welfare.