纤维素酶的筛选和葡萄糖耐受性β-葡萄糖苷酶的初步优化生产和表征。

IF 4.6 2区 生物学 Q1 MYCOLOGY Mycology Pub Date : 2023-01-01 DOI:10.1080/21501203.2022.2155261
Nivisti Singh, Bruce Sithole, Roshini Govinden
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引用次数: 3

摘要

寻找一种新的纤维素酶微生物生产者包括葡萄糖耐受性β-葡萄糖苷酶是一个挑战,因为大多数被其产物葡萄糖抑制。本研究旨在利用定性和定量筛选方法筛选纤维素分解真菌。初步筛选发现46株真菌中有34株具有β-葡萄糖苷酶活性。其中11个和13个分别表现出内切葡聚糖酶和外切葡聚糖酶活性。在二次筛选时,这一数量减少到26个β-葡萄糖苷酶生产者,其中13个还具有内切葡聚糖酶和外切葡聚糖酶活性。分离物C1在0.05 M葡萄糖的存在下显示出β-葡萄糖苷酶的增强(活性提高69%)。通过单变量优化β-葡萄糖苷酶的生长条件,提高了(分离株)PS1(64%)、MB5(84%)和C2(69%)的产量。分离物PS1鉴定为Chaetomella sp. BBA70074对葡萄糖的耐受性最高,在0.8 M葡萄糖存在下保持10%的β-葡萄糖苷酶活性。在最佳条件下生产时,对葡萄糖的耐受性提高到14%。β-葡萄糖苷酶分子量为170 kDa,最适pH为6℃,最适温度为70℃。未来的研究将包括优化毛霉sp. BBA70074的葡萄糖耐受性酶的生产。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Screening for cellulases and preliminary optimisation of glucose tolerant β-glucosidase production and characterisation.

The search for a novel microbial producer of cellulases including a glucose tolerant β-glucosidase is a challenge as most are inhibited by their product glucose. This study aims to screen for cellulolytic fungi using qualitative and quantitative screening methods. Primary screening revealed 34 of 46 fungal isolates with β-glucosidase activity. Eleven and 13 of these also displayed endoglucanase and exoglucanase activities, respectively. During secondary screening, this number was reduced to 26 β-glucosidase producers with 13 also having endoglucanase and exoglucanase activities. Isolate C1 displayed enhanced production of β-glucosidases in the presence of 0.05 M glucose (69% higher activity). Optimisation of growth conditions for β-glucosidase production by one variable at a time experiments improved production for (isolates) PS1 (64%), MB5 (84%), and C2 (69%). Isolate PS1 identified as Chaetomella sp. BBA70074 displayed the highest tolerance to glucose, retaining 10% of β-glucosidase activity in the presence of 0.8 M glucose. Tolerance to glucose increased to 14% when produced under optimal conditions. β-Glucosidase had a molecular weight of 170 kDa with a pH and temperature optima of 6 and 70°C, respectively. Future studies will include optimisation of the production of the glucose tolerant enzyme by Chaetomella sp. BBA70074.

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来源期刊
Mycology
Mycology Medicine-Infectious Diseases
CiteScore
9.10
自引率
0.00%
发文量
18
审稿时长
13 weeks
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