MiR-129-2-3p通过靶向DNMT3B抑制食管癌细胞增殖、迁移和侵袭

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Current molecular pharmacology Pub Date : 2023-01-01 DOI:10.2174/1874467215666220308122716

Xuyang Peng, Xuhui Wu, Gongzhi Wu, Chongxiong Peng, Bin Huang, Mingjiang Huang, Jianyang Ding, Chaofan Mao, Huaizhong Zhang

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引用次数: 5

摘要

目的:探讨miR-129-2-3p在食管癌(EC)细胞进展中的调控机制，为EC的靶向治疗提供新思路。方法:利用TCGAESCA数据集中EC成熟miRNA表达数据和总RNA测序数据，探索差异表达miRNA (DEmiRNAs)。然后利用StarBase数据库预测miRNA的靶标。采用qRT-PCR和Western blot检测EC细胞株中MiR-129-2-3p和DNMT3B的表达。通过CCK-8、划痕愈合和transwell实验来评估miR-129-2-3p对EC细胞表型的影响。此外，我们完成了双荧光素酶测定，以确定DNMT3B与miR-129-2-3p之间的结合关系。结果:MiR-129-2-3p在EC细胞系中明显低表达，而DNMT3B则高表达。MiR-129-2-3p可以与DNMT3B结合。此外，体外功能实验发现，过表达miR-129-2-3p可抑制EC细胞的进展，而进一步过表达DNMT3B可恢复上述抑制作用。结论:MiR-129-2-3p在EC细胞中具有抑癌作用，可靶向DNMT3B，从而阻碍EC细胞的进展。

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MiR-129-2-3p Inhibits Esophageal Carcinoma Cell Proliferation, Migration, and Invasion via Targeting DNMT3B.

Purpose: The study aims to explore the regulatory mechanism of miR-129-2-3p underlying esophageal carcinoma (EC) cell progression and generate new ideas for targeted treatment of EC.

Methods: Mature miRNA expression data and total RNA sequencing data of EC in the TCGAESCA dataset were utilized to explore differentially expressed miRNAs (DEmiRNAs). StarBase database was then utilized to predict targets of miRNA. MiR-129-2-3p and DNMT3B expression in EC cell lines was assayed through qRT-PCR and Western blot. CCK-8, scratch healing, and transwell assays were conducted to assess the impact of miR-129-2-3p on EC cell phenotypes. In addition, a dual-luciferase assay was completed to identify the binding relationship between DNMT3B and miR-129-2-3p.

Results: MiR-129-2-3p was noticeably less expressed in EC cell lines, while DNMT3B was highly expressed. MiR-129-2-3p could bind to DNMT3B. Furthermore, in vitro functional experiments uncovered that overexpressed miR-129-2-3p repressed EC cell progression while further overexpressing DNMT3B would restore the above inhibitory effect.

Conclusion: MiR-129-2-3p is a cancer repressor in EC cells, and it could target DNMT3B, thus hampering the progression of EC cells.

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来源期刊

Current molecular pharmacology Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

4.90

自引率

3.70%

发文量

112

期刊介绍： Current Molecular Pharmacology aims to publish the latest developments in cellular and molecular pharmacology with a major emphasis on the mechanism of action of novel drugs under development, innovative pharmacological technologies, cell signaling, transduction pathway analysis, genomics, proteomics, and metabonomics applications to drug action. An additional focus will be the way in which normal biological function is illuminated by knowledge of the action of drugs at the cellular and molecular level. The journal publishes full-length/mini reviews, original research articles and thematic issues on molecular pharmacology. Current Molecular Pharmacology is an essential journal for every scientist who is involved in drug design and discovery, target identification, target validation, preclinical and clinical development of drugs therapeutically useful in human disease.