MiR-30a-5p通过调节CBX2抑制食管癌症细胞行为

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis Pub Date : 2023-01-01 DOI:10.1016/j.mrfmmm.2023.111818

Luxing Peng, Xinjun Huang, Defeng Qing, Heming Lu, Xu Liu, JiaXin Chen, Xianfeng Long, Qiang Pang

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引用次数: 0

摘要

背景研究了miR-30a-5p/CBX2轴对食管癌症（EC）的影响。方法利用癌症基因组图谱数据库确定研究对象。通过qRT-PCR、蛋白质印迹、双荧光素酶报告基因、MTT、Transwell和伤口愈合方法，我们测试了基因表达和不同的细胞行为。miR-30家族成员靶向CBX2并抑制CBX2的表达。miR-30a-5p/CBX2轴可抑制EC细胞的行为。结论MiR-30a-5p为EC的治疗提供了新的启示。

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MiR-30a-5p inhibits cell behaviors in esophageal cancer via modulating CBX2

Background

This investigation studied the impacts of the miR-30a-5p/CBX2 axis on esophageal cancer (EC).

Methods

Research objects were ascertained using The Cancer Genome Atlas database. Followed by qRT-PCR, western blot, dual-luciferase reporter, MTT, Transwell, and wound healing approaches, we tested gene expression and varying cell behaviors

Results

Conspicuously miR-30 family members (miR-30a-5p, miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-30e-5p) downregulation and CBX2 upregulation were discovered in EC cells. miR-30 family members target CBX2 and inhibited CBX2 expression. EC cell behaviors were inhibited by miR-30a-5p/CBX2 axis.

Conclusion

MiR-30a-5p draws a new inspiration for EC treatment.

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来源期刊

Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 生物-毒理学

CiteScore

4.90

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Mutation Research (MR) provides a platform for publishing all aspects of DNA mutations and epimutations, from basic evolutionary aspects to translational applications in genetic and epigenetic diagnostics and therapy. Mutations are defined as all possible alterations in DNA sequence and sequence organization, from point mutations to genome structural variation, chromosomal aberrations and aneuploidy. Epimutations are defined as alterations in the epigenome, i.e., changes in DNA methylation, histone modification and small regulatory RNAs. MR publishes articles in the following areas: Of special interest are basic mechanisms through which DNA damage and mutations impact development and differentiation, stem cell biology and cell fate in general, including various forms of cell death and cellular senescence. The study of genome instability in human molecular epidemiology and in relation to complex phenotypes, such as human disease, is considered a growing area of importance. Mechanisms of (epi)mutation induction, for example, during DNA repair, replication or recombination; novel methods of (epi)mutation detection, with a focus on ultra-high-throughput sequencing. Landscape of somatic mutations and epimutations in cancer and aging. Role of de novo mutations in human disease and aging; mutations in population genomics. Interactions between mutations and epimutations. The role of epimutations in chromatin structure and function. Mitochondrial DNA mutations and their consequences in terms of human disease and aging. Novel ways to generate mutations and epimutations in cell lines and animal models.